Mara Esposito

6 Questions 4 Answers 0 Followers

Questions related from Mara Esposito

What does indicate the following FACS data about PMNs infection?

Dear all, I performed a PMN's infection using a pathogen. I analyzed the infected cells using flow cytometry. The infection was performed for 1 and 6 hpi. I have attached the data to the...

30 October 2023 3,688 0 View

Can the hematology analyzer be used for fixed blood samples?

Hi, I have a question regarding the hematology analyzer for complete blood count for blood samples. I would need to analyze fixed blood samples and I would like to know if the analyzer could work...

12 August 2021 360 3 View

Fixation of Brucella for Flow Cytometry?

I need to do fixation of blood samples infected with Brucella to sort those samples by FACS and later to use them for genomic studies. As I learnt PFA interfere with for example proteomic...

03 June 2021 9,187 0 View

How do you do the normalisation of X protein to study the effect of a protein Y mutation on X protein level by western blot?

I need to study the effect of the protein Y mutation on the levels of a protein X. So I over-expressed the protein X under 5 conditions: (1) not over-expression of protein Y (2) with...

08 June 2017 5,361 4 View

Is it possible to load 120 ug of proteins in a protein gel?

I need to load 120 ug of proteins in a protein gel. Do I need some precaution for the protein migration and western-blot?

12 July 2016 756 4 View

High variability in western blot, why?

I am trying to detect endogenous Aurora B in the whole cell extract but I have high variability during the detection by LiCOR scanner. I use 2 different primary antibodies from BD technology and...

11 December 2015 4,091 6 View