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Questions related from Mara Esposito
Dear all, I performed a PMN's infection using a pathogen. I analyzed the infected cells using flow cytometry. The infection was performed for 1 and 6 hpi. I have attached the data to the...
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Hi, I have a question regarding the hematology analyzer for complete blood count for blood samples. I would need to analyze fixed blood samples and I would like to know if the analyzer could work...
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I need to do fixation of blood samples infected with Brucella to sort those samples by FACS and later to use them for genomic studies. As I learnt PFA interfere with for example proteomic...
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I need to study the effect of the protein Y mutation on the levels of a protein X. So I over-expressed the protein X under 5 conditions: (1) not over-expression of protein Y (2) with...
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I need to study the cell cycle using PI by FACS. I would like to know if the difference in the cell number between samples can affect the FACS analysis. In the scatter plot (Cell count vs PI) the...
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I need to load 120 ug of proteins in a protein gel. Do I need some precaution for the protein migration and western-blot?
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It will be my first time that I work with Glioblastoma cell lines. I would like to know which are the important difference at genetic level between U87-MG and A-172 cell lines and how they...
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I am trying to detect endogenous Aurora B in the whole cell extract but I have high variability during the detection by LiCOR scanner. I use 2 different primary antibodies from BD technology and...
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