CBE, gRNA, CRISPR Cas9 nickase
Hey, check out 4 this!! it will give you some tips
https://academic.oup.com/nar/article-abstract/50/7/4161/6555426
https://www.mdpi.com/1580212
Article CRISPR-Cas9 mediated genome tailoring to improve nutritional...
https://www.nature.com/articles/s41467-022-30514-1
How can a relationship between two people be measured?
24 July 2024 7,357 3 View
That's the title of an essay I have to write in qualitative methods For my PHD. As I love to include individual perspectives like an ethnographic perspective into the academic field to it, I want...
20 April 2024 5,030 3 View
I need PDF books or articles that were written on biodiversity conservation?
29 February 2024 8,914 1 View
What is the breakdown rate of naphthalene in ambient air? Scources of naphtaleen e.g. burning of wood or tabacco, production of asphalt.
18 September 2023 1,215 0 View
I would like to publish an article
27 July 2023 7,780 2 View
SNP Genotyping Allele
17 November 2022 4,394 1 View
Measuring methane from ruminants is key for estimating breeding values for reduced emissions and for looking at responses to methane mitigation treatments. What is the best methods, equipment and...
23 June 2022 6,240 3 View
Does anyone know if there has been any work done to evaluate the process of writing research proposals? (e.g. for European H2020 projects)? Effort? Costs? Average acceptance rates?
27 January 2022 3,641 3 View
Home office during the Corona pandemic has changed the workflow of project management in our research group - things have become much more cumbersome. Therefore we are looking for a project...
10 March 2021 1,131 8 View
Which software is best for Structural equation modeling (SEM) analysis?
07 March 2021 5,568 2 View
I have been running a MAGeCK test command on the terminal for a CRISPR screen to rank sgRNAs and genes based on the read count tables. However, I get only the plot of the top-ranked genes...
28 July 2024 9,811 1 View
Why knockouts for same genes from two different gRNA are showing different result? I followed CRISPR-CAS9 gene editing technology.
25 July 2024 8,313 1 View
Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...
22 July 2024 7,073 2 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
I have attempted various methods, including homozygous gene disruption and CRISPR-Cas9, but have not achieved the desired results. It appears that the gene of interest is situated in a...
15 July 2024 3,977 1 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View
I am looking for a CRISPR protocol for application in leishmania strains
08 July 2024 9,732 1 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...
13 June 2024 9,473 0 View
I am currently investigating the CRISPR 9 case to target my gene of interest for knockout. The vector being used is lentiCRISPR v2puro. I digested and dephosphorylated 5ug of the lentiviral...
03 June 2024 2,877 7 View