How can I separate Gertrud Pfister's profile and the profile of Gerald Gems?

we are co-authors of several publications. But I am the only and single author of most of my publications which are based on my own research. How can I separate the profiles?

21 February 2021 4,582 3 View

I want to do research on the environmental diversity between the space outside and inside of the building and how it affects the design process?

I'm not sure specifically about the topics. It's just generalized and I need to filter it down to one topic. Any suggestions on topics and books?

15 November 2020 4,288 3 View

What is a valid and reliable instrument to measure/check the health literacy of the non professionals concerning chronic back pain?

13 April 2020 8,843 2 View

Is it possible to search for a specific pixel value within a raster frame and extract them at the same time?

Hello, I am looking for a method/s where the DN value of a pixel is available and will be used as an input for a query to search the whole raster frame for similar DN value. Do you have any...

02 January 2020 6,124 3 View

What is the best way to evaluate the science excellence of a research proposal?

Science Excellence is a key criteria needed in the evaluatation of reseach projects. What are the key factors that need to be assessed and their relative importance. How is this assessement best...

25 December 2019 5,028 6 View

Can I mix trypticase soy broth and nutrient agar together?

I am a non-microbiologist doing some microbiology work. I have plenty of dehydrated media for both nutrient agar and trypticase soy broth. If I need to make some trypticase soy agar, without...

12 November 2019 2,893 6 View

App to collector smartphone data?

In the course of a research project we want to use smartphone data to optimize the energy consumption of buildings; the idea is to install a "collector app". Can you recommend a flexible app...

20 October 2019 9,485 3 View

What is the maximum time a specimen can stay in formalin 10% before pathologist analysis?

I have specimens stored in Formalin 10% for more than 6months.( Due to no resident pathologist in Country). Could they still be processed for His to pathology?

02 October 2019 1,467 7 View

Does anybody have experience in using the AGILE and SCRUM approaches to doing agricultural science and what where the outcomes?

science processes need to evolve with the rapid pace of science advances. SCRUM and AGILE processes long used in IT.

30 January 2019 9,912 2 View

What can I do if the wrong person appears on my profile ?

If I search for my profile - name - on research gate the name of a colleague appears and our two profiles seem to be intertwined maybe because he used my computer to create his profile ?

19 October 2018 9,222 2 View

In what ways do fellow reaerchers think that mRNA vaccination may be under CRISPR systems technology application ?

There are many ways for mRNA to intervene with genome expression. This question is raised for experts in CRISPR systems. Any RNA does not stand alone within acell. There are many ways to become...

18 February 2021 9,109 5 View

How can I completely delete a protein on the genomic level using Crispr?

I want to completely delete or largely truncate a gene and not only achieve a functional knockout in a cell line. I can only transfect my cells with the Crispr/Cas9 sgRNA RNP and not introduce a...

08 February 2021 2,032 2 View

How to model Type 1 collagen structure for finite element analysis ?

I have started a project in which stress distribution in Type 1-collagen scaffold is to be modelled. I am thinking of using finite element analysis to model the structure and the loading...

07 February 2021 5,487 3 View

Choise of cell line for neurobiology?

I am working with myelin-related proteins and interested to do cell experiments with my target protein in cells. I have created lentiviruses for my proteins, and mutants and also CRISPR set-up for...

02 February 2021 8,083 1 View

Does gene insertion via CRISPR/Cas9 result in chromosomal abnormalities?

Hello, I hope to insert a gene into mammalian cells in culture using CRISPR/Cas9 and an HDR template. Will insertion of a 1 kilobase gene cause chromosomal abnormalities and effect cell...

31 January 2021 2,516 1 View

Does anyone has a protocol for transfecting AML12 cells?

I am optimizing a CRISPR/CAS9 system in vitro before using it in vivo. I would like to transfect AML12 cells with my pAAV-sgRNA-vector to validate editing efficiency before generating virus.

26 January 2021 8,733 2 View

Why sometimes current- voltage plot in chemiresistors/ FET does not pass through the origin?

I am trying to measure resistance from I-V curve from a chemiresistor/ FET sensor with a keithly sourcemeter. The substrate is soaked/ wet with protein and phosphate buffer only. But the I-V line...

24 January 2021 8,009 3 View

Should I add a promoter upstream of my CRISPR-inserted gene?

Hello, I want to insert a gene into my cells using sgRNA, Cas9, and an HDR template. The gene that I will insert into has its own promoter. Because I will cut the gene downstream of its start...

14 January 2021 1,170 2 View

Does anyone have problems performing CRISPR KO in human primary T cells?

For the past few months, I have been troubleshooting a CRISPR KO of human CD3e in human primary T cells. I got the KO to work one time using 1250 ng TrueCut Cas9 (Thermo) + 250 ng (or 7.5 pmol)...

11 January 2021 6,016 3 View

After CRISPR and single-colony picking, cells reverted to WT?

Hi guys: I have been doing CRISPR knock-in and knock-out and one seemingly impossible scenario keeps happening. In the CRISPR knock-in attempt I tried to add a tag on a gene of interest, so I...

07 January 2021 190 3 View