How can I separate Gertrud Pfister's profile and the profile of Gerald Gems?

we are co-authors of several publications. But I am the only and single author of most of my publications which are based on my own research. How can I separate the profiles?

21 February 2021 4,582 3 View

I want to do research on the environmental diversity between the space outside and inside of the building and how it affects the design process?

I'm not sure specifically about the topics. It's just generalized and I need to filter it down to one topic. Any suggestions on topics and books?

15 November 2020 4,288 3 View

What is a valid and reliable instrument to measure/check the health literacy of the non professionals concerning chronic back pain?

13 April 2020 8,843 2 View

Is it possible to search for a specific pixel value within a raster frame and extract them at the same time?

Hello, I am looking for a method/s where the DN value of a pixel is available and will be used as an input for a query to search the whole raster frame for similar DN value. Do you have any...

02 January 2020 6,124 3 View

What is the best way to evaluate the science excellence of a research proposal?

Science Excellence is a key criteria needed in the evaluatation of reseach projects. What are the key factors that need to be assessed and their relative importance. How is this assessement best...

25 December 2019 5,028 6 View

Can I mix trypticase soy broth and nutrient agar together?

I am a non-microbiologist doing some microbiology work. I have plenty of dehydrated media for both nutrient agar and trypticase soy broth. If I need to make some trypticase soy agar, without...

12 November 2019 2,893 6 View

App to collector smartphone data?

In the course of a research project we want to use smartphone data to optimize the energy consumption of buildings; the idea is to install a "collector app". Can you recommend a flexible app...

20 October 2019 9,485 3 View

What is the maximum time a specimen can stay in formalin 10% before pathologist analysis?

I have specimens stored in Formalin 10% for more than 6months.( Due to no resident pathologist in Country). Could they still be processed for His to pathology?

02 October 2019 1,467 7 View

Does anybody have experience in using the AGILE and SCRUM approaches to doing agricultural science and what where the outcomes?

science processes need to evolve with the rapid pace of science advances. SCRUM and AGILE processes long used in IT.

30 January 2019 9,912 2 View

What can I do if the wrong person appears on my profile ?

If I search for my profile - name - on research gate the name of a colleague appears and our two profiles seem to be intertwined maybe because he used my computer to create his profile ?

19 October 2018 9,222 2 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View