SNP
Genotyping
Allele
Gerald Zvobgo that would depend upon the type of technique you want to use. Taqman, ARMS-PCR, RFLP all assays require different type of primers.
How can a relationship between two people be measured?
24 July 2024 7,357 3 View
That's the title of an essay I have to write in qualitative methods For my PHD. As I love to include individual perspectives like an ethnographic perspective into the academic field to it, I want...
20 April 2024 5,030 3 View
I need PDF books or articles that were written on biodiversity conservation?
29 February 2024 8,914 1 View
What is the breakdown rate of naphthalene in ambient air? Scources of naphtaleen e.g. burning of wood or tabacco, production of asphalt.
18 September 2023 1,215 0 View
I would like to publish an article
27 July 2023 7,780 2 View
CBE, gRNA, CRISPR Cas9 nickase
13 November 2022 3,095 1 View
Measuring methane from ruminants is key for estimating breeding values for reduced emissions and for looking at responses to methane mitigation treatments. What is the best methods, equipment and...
23 June 2022 6,240 3 View
Does anyone know if there has been any work done to evaluate the process of writing research proposals? (e.g. for European H2020 projects)? Effort? Costs? Average acceptance rates?
27 January 2022 3,641 3 View
Home office during the Corona pandemic has changed the workflow of project management in our research group - things have become much more cumbersome. Therefore we are looking for a project...
10 March 2021 1,131 8 View
Which software is best for Structural equation modeling (SEM) analysis?
07 March 2021 5,568 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...
25 July 2024 6,517 3 View
Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...
23 July 2024 1,329 5 View
Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...
20 July 2024 5,405 2 View
How to assign allele numbers and sequence type after amplifying housekeeping genes by PCR for genotyping of S. pneumoniae strains by MLST using bioedit ?
19 July 2024 5,239 2 View