I reconstituted a vial of TGF-beta from Sigma that was 10UG concentration by adding 0.1% BSA with sterile water (so I added 100ul of it). How do you reconstitute TGF-beta? Help appreciated!
For reconstitute TGF-Beta it can be purified recombinant human TGF-beta 1as an extremely hydrophobic protein that adheres strongly to surfaces. For conformation its recovery it can be reconstituted at 20 μg/mL in sterile 4 mM HCl containing 1 mg/mL human or bovine serum albumin.