I need to make the concentration to 5mg/ml from 30mg/ml but i am confused as my initial volume of 500 microlitre of 30mg/ml decrease to volume 100 microlitre .do the concentration remains 30mg/ml in decreased volume also?
No, the concentration of the plant extract will not be the same if the volume decreases from 500 microliters to 100 microliters while keeping the initial concentration constant at 30 mg/ml.
To calculate the new concentration after the volume change, you can use the formula for concentration:
So, when the volume decreases from 500 microliters to 100 microliters while maintaining the initial concentration of 30 mg/ml, the new concentration will be 150 mg/ml.
NOTE= "If there were no physical factors involved, or you can prepare a fresh solution."