Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

Which root in this tap root system, in the picture attached, should be used for root sectioning in order to study the root anatomical details?

There are numerous roots in this tap root system. Kindly suggest or share a research paper where I can clarify the doubts as to which root should be chosen for sectioning and for further root...

14 February 2021 1,816 3 View

Hi, i am working on PARP inhibitors poorly water soluble but soluble in toxic solvents acetonitrile / DMSO, please suggest nontoxic solvents ?

Drug is poorly water soluble and soluble in solvents like acetonitile , DMSO, mixture of methanol and water but they are toxic. kindly suggest any safe solvent.

18 September 2020 5,128 4 View

How do I conclude if my gene has low expression based on the results obtained?

Hello all, I am working on genes affected in cholesterol metabolism in patients with Coronary Artery Disease and PCSK9 is one such target gene in my study. I have managed to obtain stable results...

13 June 2020 7,074 9 View

What is the institute/organization that provides training for hydroponics development ?

I also want to know the organization working on development of nutrient solution for hydroponics and the organization providing training on it.

02 June 2020 8,901 4 View

Can you please help me to explain, why this attached SEM images show different effect of independent variable to dependent variables of same data?

Both Fig 1 and 2 describe aboveground biomass (AGB) and conducted SEM. However, the results were inconsistent. In fig.1, Nitrogen has significantly negative effect on AGB while in fig. 2, it was...

07 May 2020 6,280 3 View

What method can I use to analyse the language on Social media?

I'm doing a comparative study on social media language used by native and non-native speakers with special reference to Instagram. I am planning on using Discourse analysis. What is your take on...

18 April 2020 300 25 View

In biosorption process (Lantana camara leaves as biosorbent), why do we get a precipitate after putting the filtrate on hot plate for agitation?

Usually, we get a clear filtrate after performing batch experiments; same is with Lantana indica leaves as biosorbent. But, after filtration we observe dark precipitation in this case. Is it due...

03 April 2020 9,406 1 View

Can we get 20 ensembles as output from NMR structures after energy minimization from YASARA ?

I am uploading 20 ensembles in the single PDB file, after energy minimization I just got one PDB structure. Is there any possibility to get 20 ensembles out?

23 March 2020 4,599 3 View

Is it economically feasible to produce biochar at industrial scale in developing economies?

In the context when majority of studies undertaken in various parts of worlds have reported beneficial effects of biochar to enhance agricultural productivity, governments in developing countries...

27 February 2020 8,426 11 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

How do I change g/l into mM for bacto agar. I am trying to get the millimolar content of sodium and chloride percentage in bacto agar. How do I do it?

I am doing a salt tolerance-related experiment and for that I need to relate my salt levels to that in bacto agar. I don't want my salt concentrations to increase due to already salt content...

09 February 2021 4,158 1 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View

What is the composition of the solid and broth agar to grow Streptomyces from freezed sample? what are extraction steps of products from broth agar?

Please what are the composition of the solid agar could I use to grow the bacteria? what are the composition of the broth agar could I use to grow the bacteria and how to extract the natural...

26 January 2021 5,006 1 View