Hello All!
I am currently trying to use Brookhaven Instrument's Nanobrook Omni to measure zeta potential of a protein I'm working with. I was wondering if there are special guidelines if any for measuring zeta potentials for proteins with a high polydispersity index. Most work done on measuring zeta potenial of proteins involves model proteins such as BSA and lysozyme. Since I am new to this field, any help and advice is much appreciated!
Best,
Ashutosh
Hi Ashutosh,
The method which I have used for measuring the zeta potential of protein solutions is by electrophoretic mobility. I believe that the Brookhaven Instrument's Nanobrook Omni works on this principle. The equipment that I used was the Zetasizer Nano Series (Malvern Instruments, UK).
The method is described in the following article:
http://www.sciencedirect.com/science/article/pii/S0927775715301448
I believe there is no special method for proteins with a high PDI. As long as the concentration of protein is sufficiently dilute (typically ~ 0.1 wt.% solids) to allow measurement, it should be fine. Furthermore, the software should offer indications as to whether the measurement is of good quality.
Hope this helps.
Jonathan
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