Ashutosh S Phadte

4 Questions 10 Answers 0 Followers

Questions related from Ashutosh S Phadte

Clear coli Bl21(DE3) cells for production of recombinant proteins?

Hello all, I am currently reading up on clear coli BL21(DE3) cells as a possible alternative to BL21(DE3) cells for recombinant protein production such that my end products are not contaminated by...

08 August 2019 2,920 3 View

How do we measure zeta potential of proteins??

Hello All! I am currently trying to use Brookhaven Instrument's Nanobrook Omni to measure zeta potential of a protein I'm working with. I was wondering if there are special guidelines if any for...

05 May 2016 7,625 1 View

How can I use a CRISPR-Cas9 system to generate a point mutation in cell culture?

Hello! I was reading up on the using the CRISPR-Cas9 system to introduce a point mutation in a protein I work with, and I had a couple of queries: Do I need to have two plasmids - one with the...

01 January 2015 7,751 3 View

Does anyone have experience with FRET?

I'm trying to utilize protein labeling with alexa fluor dyes in order to calculate the rate of subunit exchange between two oligomeric proteins known to interact with each other. I've labelled one...

09 September 2014 1,011 4 View