How do we can quantify free radicals concentration in cell culture media?

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Yuanyuan Liu

I never quantify RNS, but I have some experience with ROS. Can you specify what ROS you want to quantify? H2O2, O2 or hydroxyl radicals?

Meysam Khosravi Farsani

Dear Yuanyuan

I'm going to quantify total ROS and RNS in culture media including H2O2, O2, Hydroxyl radicals and so on.

Sabbir Khan

Dear Meysam Khosravi Farsani,

You can try the fellow paper method in experiment.

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1574951/

Dear Sabbir

I would like to appreciate you for this useful paper.

Meysam