After oxidizing HSA with different oxidants like H2O2. I'm going to know normal range of thiol concentration for native protein (mol Thiol/mol Protein) as we have just one Cysteine with a free Thiol group on HSA.
After oxidizing protein solution with different oxidants I added 50 micro liter of protein solution into 950 micro liter DTNB solution,prepared in phosphate buffer, and finally I reached to 0.4mol/mol thiol concentration for control group. I wonder whether I have a normal ratio or not.
Meysam
The normal range of thiol (-sH) in human serum albumin is 500-600 micromoles/L
Dear Hossam
As you know we have only one free Thiol group in HSA and other SH groups (17) have involved in disulfide bonds. I wonder if you mean free Thiol group concentration in native protein or in reduced state of HSA with DTT or 2-ME. you can read the following paper.
Meysam
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