What are the excitation and emission wavelengths of Propidium Monoazide dye?

Can one take the excitation and emission wavelengths of Propidium Iodide for PMA (since it is the derivative of PI only). If not, then what is the exact excitation and emission spectrum of PMA?

05 June 2014 6,736 3 View

What is the effect on signal generation when two molecular beacons in open state are placed together?

In Molecular beacon based qPCR assays, when the probe-target hybridization takes place, do quenching occur between the fluorophore of one molecular beacon molecule (in open state) and quencher of...

04 May 2014 8,277 3 View

PMA based real- time PCR for the differentiation of dead and live bacteria?

I am using PMA (Propidium monoazide) dye for the differentiation of live and dead bacteria. Ideally, on treating dead bacteria with PMA, one should not get any amplification in the reaction tube...

04 May 2013 6,966 9 View

Can anyone help me in clarifying my doubts regarding reproducibility of LAMP PCR?

I am working on LAMP PCR. The problem is, that initially I was getting positive results for my samples but suddenly I am not getting any amplification in reaction tubes for the same DNA? I do not...

04 May 2013 3,802 6 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View