How do miRNA reaches blood plasma. Please share some references explaining this phenomenon.
Can one take the excitation and emission wavelengths of Propidium Iodide for PMA (since it is the derivative of PI only). If not, then what is the exact excitation and emission spectrum of PMA?
05 June 2014 6,756 3 View
In Molecular beacon based qPCR assays, when the probe-target hybridization takes place, do quenching occur between the fluorophore of one molecular beacon molecule (in open state) and quencher of...
04 May 2014 8,296 3 View
I am using PMA (Propidium monoazide) dye for the differentiation of live and dead bacteria. Ideally, on treating dead bacteria with PMA, one should not get any amplification in the reaction tube...
04 May 2013 6,990 9 View
I am working on LAMP PCR. The problem is, that initially I was getting positive results for my samples but suddenly I am not getting any amplification in reaction tubes for the same DNA? I do not...
04 May 2013 3,830 6 View
Census data seems only to reach the year 2011, but there might be estimates somewhere that I cannot find? I am looking not only for the largest cities but also towns etc. Thanks!
10 August 2024 857 4 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
I have reached out to the original creator Dr. Demerouti but have had no luck with a response. I am completing my IRB form and need the information or permission by tomorrow. I know short notice,...
29 July 2024 3,684 0 View
I am using the Polyomics Integrated Metabolomics Pipeline (PiMP) for metabolite feature analysis. After annotating my data, I have encountered cases where multiple potential metabolites are...
24 July 2024 5,789 0 View
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
24 July 2024 9,337 3 View