Hello Neetika,

for PMA: Abs/Em = 510/610 nm (http://biotium.com/product/pmatm-dye-propidium-monoazide/)

for PI: Abs/Em = 536/617 nm (http://www.olympusmicro.com/primer/techniques/fluorescence/fluorotable2.html)

Best regards

Here are the details Nikita

Name : PMATM, Propidium Monoazide

Phenanthridium, 3-amino-8-azido-5-[3-

(diethylmethylammonio)propyl]-6-

phyenol dichloride

Catalog Number : FP-BZ9340, 1 mg

Structure : C27H33Cl2N6

Molecular Weight : MW= 512.51

Solubility: Water, DMSO or DMF at least 10mg/ml

Absorption / Emission : λabs (before photolysis) = 464 nm

λexc./λem(following photolysis and covalent

attachment to DNA/RNA)= 510/610nm

@Christian Q. Scheckhuber and Deepti: Thanks for the valuable answer.