I want to study the effect of non host protein on B cell subset, so can I directly inject this protein in mouse iv and then do immunophenotypying of spleen by flow cytometry in comparision to B cells fom albumin protein injected in mice.
Thank you
Komal
Yes, you can inject iv and analyze the surface phenotype of the splenic B cells. What are you specifically looking for? Up-regulation of activation markers? Or are you anticipating depletion of a particular B cell subset (FO vs MZ)?. Your approach seems sound; however, I would caution you to verify the absence of TLR agonists or other associated PAMPs with your proteins because they may confound your result. Best of luck!
@ Greg A Kirchenbaum,
Thank you Sir for the answer.
I am anticipating some change in B cell subset.
Do you anticipate an increase or decrease in transitional (T1/T2) B cells, or an expansion/contraction of FO vs MZ? What markers are you intending to use to discriminate the splenic B cell compartments?
@ Greg A Kirchenbaum
I will just be looking at change in percentage of Immature, mature, activated, plasma and memory B cell population in spleen by using marker antibodies specific for each subset and not for the compartment.
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