Komal Dolasia

11 Questions 9 Answers 0 Followers

Questions related from Komal Dolasia

what range of blood dilution should be used to determine CFU in CLP mice?

Hello, I want to determine infection load in CLP mice after 24 hours from blood and peritoneal lavage. In BALB/C, I have ligated 75 % cecum and punctured the cecum twice with 18 G needle , then...

26 October 2017 5,098 0 View

For CLP model of sepsis in mice while using antibiotics why Ceftriaxone is always used with some other antibiotics and not alone?

While using antibiotic Ceftriaxone in mice for CLP model of sepsis, it is always used with some other antibiotics like metronidazole or clindamycin and not alone. Can't we use ceftriaxone alone...

19 August 2017 6,603 2 View

Inhibition of protein but not of peptide antigen presentation, what does it imply?

I have a compound that inhibits MHC class II antigen presentation of OVA protein but not of OVA peptide by peritoneal macrophages, does this mean that it inhibits the antigen processing or...

19 August 2017 168 11 View

Are PBMC isolated from blood collected in ACD blood bag culturable?

I just heard from someone that PBMC isolated from blood collected in ACD blood bag can not be cultured, is it true?

27 March 2017 6,741 1 View

How to prevent/avoid Blood loss while cutting peritoneal cavity of mouse?

For performing Cecal ligation and puncture in mouse, is it possible to cut open the peritoneal cavity without damaging blood vessel, so that no/minimal blood loss occurs.If yes, can someone...

03 March 2017 9,919 3 View

Cell pellet not dissolving in TRI reagent?

Pellet of single cell suspension of mouse splenocyte is not dissolving in TRI reagent even after vigorous vortexing, rather it has formed a tight mass.Earlier it used to, but this time it did...

23 November 2016 281 1 View

What is the percentage of immature, mature,marginal zone, follicular zone, activated, plasma, memory and regulatory B cells in spleen of mice?

Can someone tell the percentage of immature, mature,marginal zone, follicular zone, activated, plasma, memory and regulatory B cells in spleen of normal mice. Along with reference if...

15 August 2016 8,218 1 View

At what time point after infection should one look to see the effect of protein on various B cell subset?

I want to see the effect of non-host protein on Various B cell subset, viz, immature, mature, activated, plasma, memory and regulatory B cell subset , by infecting mice with bacteria expressing...

18 November 2015 5,411 1 View

Any advice on why no GFP overexpression in Mycobacterium Smegmatis upon gene of interest expression was observed?

I have an M. smegmatis strain with GFP in pFPV27 vector(with kanamycin resistance) under the control of Mtb rrn promoter.When I transform this bacterium with pVV 16 vector(with hygromycin and...

13 October 2015 2,802 3 View

How do I study the effect of a protein on a B-cell subset in mice?

I want to study the effect of non host protein on B cell subset, so can I directly inject this protein in mouse iv and then do immunophenotypying of spleen by flow cytometry in comparision to B...

19 April 2015 2,422 4 View

Should experiments to check the effect of oxidative stress on macrophage be done in the presence or absence of serum?

I want to check the effect of hydrogen peroxide on macrophage, and I am in dilemma whether the experiment has to be done in presence or absence of serum, as in physiological scenario serum will be...

22 June 2014 2,747 3 View