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While using antibiotic Ceftriaxone in mice for CLP model of sepsis, it is always used with some other antibiotics like metronidazole or clindamycin and not alone. Can't we use ceftriaxone alone...
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I have a compound that inhibits MHC class II antigen presentation of OVA protein but not of OVA peptide by peritoneal macrophages, does this mean that it inhibits the antigen processing or...
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Pellet of single cell suspension of mouse splenocyte is not dissolving in TRI reagent even after vigorous vortexing, rather it has formed a tight mass.Earlier it used to, but this time it did...
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Can someone tell the percentage of immature, mature,marginal zone, follicular zone, activated, plasma, memory and regulatory B cells in spleen of normal mice. Along with reference if...
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I want to see the effect of non-host protein on Various B cell subset, viz, immature, mature, activated, plasma, memory and regulatory B cell subset , by infecting mice with bacteria expressing...
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I have an M. smegmatis strain with GFP in pFPV27 vector(with kanamycin resistance) under the control of Mtb rrn promoter.When I transform this bacterium with pVV 16 vector(with hygromycin and...
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I want to study the effect of non host protein on B cell subset, so can I directly inject this protein in mouse iv and then do immunophenotypying of spleen by flow cytometry in comparision to B...
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I want to check the effect of hydrogen peroxide on macrophage, and I am in dilemma whether the experiment has to be done in presence or absence of serum, as in physiological scenario serum will be...
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