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Questions related from Komal Dolasia
Hello, I want to determine infection load in CLP mice after 24 hours from blood and peritoneal lavage. In BALB/C, I have ligated 75 % cecum and punctured the cecum twice with 18 G needle , then...
26 October 2017 5,093 0 View
While using antibiotic Ceftriaxone in mice for CLP model of sepsis, it is always used with some other antibiotics like metronidazole or clindamycin and not alone. Can't we use ceftriaxone alone...
19 August 2017 6,600 2 View
I have a compound that inhibits MHC class II antigen presentation of OVA protein but not of OVA peptide by peritoneal macrophages, does this mean that it inhibits the antigen processing or...
19 August 2017 164 11 View
I just heard from someone that PBMC isolated from blood collected in ACD blood bag can not be cultured, is it true?
27 March 2017 6,738 1 View
For performing Cecal ligation and puncture in mouse, is it possible to cut open the peritoneal cavity without damaging blood vessel, so that no/minimal blood loss occurs.If yes, can someone...
03 March 2017 9,912 3 View
Pellet of single cell suspension of mouse splenocyte is not dissolving in TRI reagent even after vigorous vortexing, rather it has formed a tight mass.Earlier it used to, but this time it did...
23 November 2016 277 1 View
Can someone tell the percentage of immature, mature,marginal zone, follicular zone, activated, plasma, memory and regulatory B cells in spleen of normal mice. Along with reference if...
15 August 2016 8,215 1 View
I want to see the effect of non-host protein on Various B cell subset, viz, immature, mature, activated, plasma, memory and regulatory B cell subset , by infecting mice with bacteria expressing...
18 November 2015 5,408 1 View
I have an M. smegmatis strain with GFP in pFPV27 vector(with kanamycin resistance) under the control of Mtb rrn promoter.When I transform this bacterium with pVV 16 vector(with hygromycin and...
13 October 2015 2,799 3 View
I want to study the effect of non host protein on B cell subset, so can I directly inject this protein in mouse iv and then do immunophenotypying of spleen by flow cytometry in comparision to B...
19 April 2015 2,418 4 View
I want to check the effect of hydrogen peroxide on macrophage, and I am in dilemma whether the experiment has to be done in presence or absence of serum, as in physiological scenario serum will be...
22 June 2014 2,743 3 View