How do I remove excess antibody after conjugating gold nanoparticles with it?

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Has anyone got a sample or an approved DST-INSPIRE faculty fellowship proposal?

I would like to get some pointers as to how DST-INSPIRE faculty fellowship proposal needs to be written. If anyone has a sample copy or an approved (old but shareable) it'd be great to get some...

21 April 2021 5,569 1 View

Are there any publications that show ECM staining of cancer microenvironment?

I am interested in knowing what happens to the ECM components like collagen and elastin in tumor environment. Can someone redirect me to publications that have actually quantified collagen and...

12 December 2018 7,953 3 View

How do I calculate number of antibody molecules bound to gold nanoparticles?

Is there a procedure or mathematical formula to calculate the number of antibody molecules bound to a gold nanoparticle? I used PEG-EDC-NHS linking chemistry to bind antibody to nanoparticle.

13 September 2017 3,602 3 View

Is there a procedure to confirm antibody tagging to gold nanoparticles?

I tried doing an SDS PAGE followed by Silver staining to stain the antibody conjuagated nanoparticles but it didn't work. Blank and antibody conjugated nanoparticles have same bands.

08 June 2017 775 4 View

Is there a 67kDa elastin binding protein present in rat species?

I have searched a lot of sources to find EBP sequence in rat. I want to know if the EBP is present in rat or if a similar protein is responsible for tropoelastin deposition in rats. The human form...

30 October 2016 9,538 1 View

EtBr not dissolving in water?

I am trying to dissolve EtBr powder in water at a concentration of 2mg/mL and I still have some residue left even after mixing well. Any suggestions on how to dissolve it completely?

14 September 2016 5,297 7 View

Can I just use nebulizer to aerosolize my already prepared nanoparticle solution?

I have a nanoparticle solution made of a biodegradable polymer and a drug inside it. The particle size in aqueous solution is around 200nm. Can I use this solution to generate aerosols for...

07 June 2016 7,249 2 View

Can I use aqueous solution of Hyaluronic acid instead of 0.25N NaOH HA solution to make hydrogels?

What is the role of NaOH in the solution while dissolving HA? Is it used to activate COOH groups and make them susceptible to crosslinking?

18 April 2016 3,221 1 View

Is there any procedure that describes how to calculate mean linear intercept using ImageJ?

I've come across papers that have used manual horizontal and vertical lines to calculate the points of intersection. There are some papers that have used complex programs to get MLI and...

08 July 2015 8,718 4 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Binder for ZnO nanoparticles in graphite paste electrode?

I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?

03 August 2024 4,624 3 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View

How gold procurement?

Please note it

29 July 2024 4,920 3 View

How gold procurement?

https://www.cleanteqwater.com/resources/case-studies/gold-mine-australia/

29 July 2024 5,161 1 View

Addition of EDTA during the synthesis of copper nanoparticles to prevent it from being oxidized?

I've attempted to use this method to synthesize copper nanoparticles. Copper nanoparticles can be synthesised using a variety of precursor materials. CuSO4, distilled water, NaOH, and EDTA are...

28 July 2024 8,027 3 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

What are the modern methods for detecting nanoparticles?

Some of the techniques I am familiar with are standard. I would like to know which of the two methods under study is preferable, as well as the cost and list of particles and devices needed in...

22 July 2024 1,921 3 View