13 December 2018 3 8K Report

I am interested in knowing what happens to the ECM components like collagen and elastin in tumor environment. Can someone redirect me to publications that have actually quantified collagen and elastin in cancers possibly with some histology?

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Has anyone got a sample or an approved DST-INSPIRE faculty fellowship proposal?

I would like to get some pointers as to how DST-INSPIRE faculty fellowship proposal needs to be written. If anyone has a sample copy or an approved (old but shareable) it'd be great to get some...

21 April 2021 5,569 1 View

How do I calculate number of antibody molecules bound to gold nanoparticles?

Is there a procedure or mathematical formula to calculate the number of antibody molecules bound to a gold nanoparticle? I used PEG-EDC-NHS linking chemistry to bind antibody to nanoparticle.

13 September 2017 3,602 3 View

How do I remove excess antibody after conjugating gold nanoparticles with it?

I conjugated gold nanoparticles with an antibody of interest and when I tried centrifuging the particles at 14000 rpm for 30 mins, I did not get a pellet. Can someone tell me what's going on? And...

08 June 2017 3,367 2 View

Is there a procedure to confirm antibody tagging to gold nanoparticles?

I tried doing an SDS PAGE followed by Silver staining to stain the antibody conjuagated nanoparticles but it didn't work. Blank and antibody conjugated nanoparticles have same bands.

08 June 2017 775 4 View

Is there a 67kDa elastin binding protein present in rat species?

I have searched a lot of sources to find EBP sequence in rat. I want to know if the EBP is present in rat or if a similar protein is responsible for tropoelastin deposition in rats. The human form...

30 October 2016 9,538 1 View

EtBr not dissolving in water?

I am trying to dissolve EtBr powder in water at a concentration of 2mg/mL and I still have some residue left even after mixing well. Any suggestions on how to dissolve it completely?

14 September 2016 5,297 7 View

Can I just use nebulizer to aerosolize my already prepared nanoparticle solution?

I have a nanoparticle solution made of a biodegradable polymer and a drug inside it. The particle size in aqueous solution is around 200nm. Can I use this solution to generate aerosols for...

07 June 2016 7,249 2 View

Can I use aqueous solution of Hyaluronic acid instead of 0.25N NaOH HA solution to make hydrogels?

What is the role of NaOH in the solution while dissolving HA? Is it used to activate COOH groups and make them susceptible to crosslinking?

18 April 2016 3,221 1 View

Is there any procedure that describes how to calculate mean linear intercept using ImageJ?

I've come across papers that have used manual horizontal and vertical lines to calculate the points of intersection. There are some papers that have used complex programs to get MLI and...

08 July 2015 8,718 4 View

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