I've come across papers that have used manual horizontal and vertical lines to calculate the points of intersection. There are some papers that have used complex programs to get MLI and Destructive Index. But is there any simpler method to do this using ImageJ, to draw lines on the image and count the points of intersection?
Hi Vaideesh,
There is not an specific way to measure the mean linear intercept, you should adapt and use your "custom" mask to evaluate. I can explain shortly what I did.
a) Load your image
b) Duplicate it (as backup)
c) Make your duplicate image Binary (Process - Binary - Make Binary)
d) Skeletonize the image ( Plugins - Skeleton - Skeletonize 2d-3d)
e) Multiply Images (Use your custom grid and multiply with the Skeletonized Image)
f) Count the intercepts (Analyze - Analyze Particles)
Remember to set the scale in order to know the distance.
For me it's the simpliest way and it only takes 2 minutes to get results!!!
Best
Juanjo
The following article presents a plugin called fraclac that may be helpful:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3680331/
Juan Jose Uriarte Hi! I an trying to calculate MLI with ImageJ following your steps. However, in the 'Plugins' dropdown menu, I couldn’t find the 'Skeleton' option. Can you please let me know which option should I follow instead?
Moreover, it would be a great help to me if you tell me where to get the result.
Nobody in my lab has used ImageJ any other way than manual counting, so it'll be a great help!
Regards,
Lira
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