How do I quantify protein extracted from cereal flour with 70% ethanol?

08 November 2019 1 4K Report

How do I quantify protein extracted from cereal flour with 70% ethanol using Lowry assay ? Standards from protein BSA can not be prepared in ethanol because of low solubility. So which is the suitable standard for ethanol solvent?

Mahesh Chandak

I havent purified protein in 70% ethanol. Does it aggregate without ethanol ?

You may allow the ethanol to evaporate from the tube at room temperature for 30 minutes or use speed-vac instrument. Do not over-dry pellet, or it may not dissolve . Then add buffer of your choice to the pallet and estimate protein concentration

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