Hello everybody.

I am looking for a protocol for histological preparation for prostate cancer.

Our technician gave me the following protocol:

Formaline 24h RT

Wash 1h in double distilled water

EtOH 70% upon parrafin inclusion

I am really worried about the quality of the samples in the end since I know that water is not "compatible" with tissue preparations since it causes osmotic shock to the samples.

And the other doubt I have is linked to moving directly to ethanol 70% without a gradient concentration. In the previous lab where I worked we used to let tissues go step by step towards ethanol or towards PBS.

Can someone suggest a better protocol or is this one I have good enough? The problem is that the samples I will have to produce are very important and I cannot lose any of them due to a bad preparation.

Thanks in advance

Elisa