Wat is the half-life of luciferase protein in mouse tissue when delivered using mRNA luciferase?

Hello, I I am validating a system to deliver mRNA using nanoparticles to a mouse model. I am initially using mRNA for firefly luciferase to be able to do live imaging in the mouse. After that, I...

17 February 2021 2,536 3 View

DoE: Possible reasons for having a lot of significant effects? Is it as complex or went something wrong?

I did a product development using Design Expert for planning and evaluation. There is a response with a lot of significant effects shown in the Half Normal Plot. By testing factors A, B, C, D I...

06 December 2020 5,128 3 View

How to know if 87Sr/86Sr is reliable for Sr isotope chemostratigraphy in marbles?

I am currently looking for parameters to know if 87Sr/86Sr data is reliable for chemostratigrahy in marbles, and/or parameters to know if those marbles have had some post-depositional...

30 November 2020 9,032 8 View

Controlling for a variable that is an outcome of the dependent variable: what is the interpretation of the coefficient of the regressor?

I am estimating a dependent variable as a function of a regressor using Ordinary Least Squares regression. If I add a control variable that is (partly) an outcome of my dependent variable, how do...

06 October 2020 3,224 1 View

Which EEG source analysis plugin for Matlab do you recommend?

Good morning, I have the EEG recordings of 65 subjects and I would like to reconstruct the source of the signal in the gamma frequency range. Unfortunately, I don't have an individual MRI or...

25 August 2020 10,021 2 View

How can I study the clusterization Vglut1 puncta in cultured cells ? Do you know exactly the dimension of one single Vglut puncta?

Hi guys, I ave to study the Vglut1 puncta density/clusterization in cultured cells and see any differences between 2 different conditions. I noticed that in treatment B Vglut puncta appear...

29 March 2020 6,120 1 View

Botany - Betula pubescens old drawing?

Does any of you have a vintage botanical print of Betula pubescens (alba) subsp. celtiberica? Thanks a lot!!!

27 February 2020 6,049 5 View

Why do I obtain wavy bands on acrylammide precast gel in western blot experiments?

Hello! I recently tested a new precast gradiented gel for my Western Blot experiments and I obtained wavy bands both for proteins and for molecular weight marker. I run in tris-glycine-sds running...

28 July 2019 2,303 6 View

Tris HCl 1.5M pH 8.8, Tris HCl 1 M pH 6.8, how to pH?

I am experiencing many problems in obtaining the correct pH of Tris-HCl solutions for acrylammide gels (pH8.8 and 6.8) due to the dependence of the pH on temperature. I prepared and brought the...

18 June 2019 7,663 9 View

Does anyone has any references for scholarly articles on teacher professional development and teacher identity?

My field research was to study the interactions of teacher leaders in a teacher leader PLC in a primary school. I am curious as to any findings you'd be willing to share and any current scholarly...

30 December 2018 8,201 3 View

How can I use Permanova to analyze individual variation with RNA seq transcripts and multiway Anova design?

I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...

13 February 2021 7,217 4 View

Should I use ANOSIM or PERMANOVA to analyses community differences within an estuary?

Hello, I am trying to decide on the best approach for statistical analyses of my data. I have never done this before and was wanting a second opinion. I am studying the impacts of nitrogen...

28 October 2020 9,978 9 View

Why do I receive the sae mesage several times?

26 October 2020 409 1 View

Confounding variable as PERMANOVA covariate: Should its inclusion be based on significance?

Hi everyone, I have an experiment investigating the effect of sediment condition (factor 1 - 2 levels) and the benthic macroinvertebrate community (factor 2 - 2 levels [faunated vs defaunated])...

11 October 2020 959 6 View

What's the connection between PERMANOVA and SIMPER?

I have a pair of groups that are not significantly different in PERMANOVA (p > 0.05), yet in SIMPER the average dissimilarity between these two groups are very high, even higher than other...

13 August 2020 8,217 2 View

Can the Bray-Curtis Dissimilarity Index show differences in relative abundances of particular species. If not, which analyses can?

Hi all, I have a dataset of fish surveys containing counts of 80 or so species seen at 11 different reefs over a several year period. I would like to compare the community composition between...

13 May 2020 9,976 12 View

I need to do a research project on how human action impacts zooplankton. Any ideas from brilliant minds?

I need to do a research project on how human action (e.g. climate change, ocean acidification, plastic, pollution, HAB, etc), impacts zooplankton and the zooplankton response. I'm struggling on...

23 March 2020 2,081 5 View

How do I best determine the energy content of gelatinous zooplankton?

Hi! I am trying to measure the energy content of various gelatinous zooplankton organisms using a semi-micro bomb-calorimeter. Because of the low expected energy content, I am mixing my sample...

19 November 2019 2,566 4 View

PerMANOVA - how to lay out species abundance data and habitat data?

For a university year 2 coursework I have a very basic dataset from using quadrats while diving to find which animals are living on coral. I also took several measurements from each quadrat...

09 October 2019 9,493 2 View

Which microparticles to use as food competitor for zooplankton?

Hi everyone, We are looking for non-toxic microparticles that could be handled and ingested by zooplankton. We would like to use them as an external perturbation impacting the functional response...

06 October 2019 2,877 1 View