I do not understand sufficiently the question. Is the oxidase secreted in an aggregated form or you want it to be secreted by the cultured microorganism?
Aggregates can be dissociated with one of the following reagents:
1) Non ionic detergents comprising Triton X-100
2) Urea at 1-2 M (the oxidase must be stable)
3) Water miscible organic solvents up to 10% comprising DMSO, isopropanol, glycerol.
4) Buffer at PH quite different of the isoelectric point of your oxidase
Please to give details for more an appropriate answer
Thank you for your answers. In detail, for the purification of cholesterol oxidase, I am culturing the organism in cholesterol enriched medium. As cholesterol isn't completely dissolved in the medium (it will be like particulate matter through out the medium) even after Tween addition, it is difficult to differentiate the growth of the bacteria and cholesterol. Again while initial ammonium sulphate purification step, it is difficult to settle it down even after centrifugation steps. That's why, I am thinking, if the medium is transparent, we can overcome some of these problems.
Please let me know your previous suggestions
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