Hi, I would like to test different disinfectants and its effects on biofilms in microplates, which paper has the best protocols!?

I need to do it only in microplates and read the OD value for the results. I cannot use any other assays except microplate. Please help me with this

07 December 2020 5,698 3 View

What is the acceptable margin error?

The calculated sample size for my study is 324 with 5% margin error. However, only 230 respondents responded the questionnaire completely. In this case, what is the margin error for this actual...

07 July 2020 524 7 View

What is another alternative to Polyvar 2 Microscope with hot plates and polarizer to check for solubility of drug in lipids?

What is another alternative to Polyvar 2 Microscope with hot plates and polarizer to check for solubility of drug in lipids? To also obtain maximum solubility reading?

02 February 2020 7,999 2 View

What is another alternative to Turbisan Lab Expert to check for nano lipid particles stability?

What is another alternative to Turbisan Lab Expert to check for nano lipid carrier's stability?

02 February 2020 9,150 2 View

XPS spectrum of CuO ?

Why the XPS spectrum of CuO doped in WO3 thin film samples show BE peaks of Cu(0) and/or Cu(I) state of copper and also Cu(II) state of copper. What is the reaction mechanism? Here the CuO doped...

26 July 2019 7,634 8 View

Why TDDFT calculation does not match with the experimental UV-Vis spectrum?

I have calculated the structure of a metal(Ag) complex for which the crystal structure data is available with us. The UV-vis spectrum of the complex shows a very strong absorption at ~250 nm and a...

08 October 2018 221 3 View

What is the difference between Factorial ANOVA and Multiple Regression?

I used same variables (3 IVs and 1 DV) to run factorial ANOVA and multiple regression. I expect the similar findings on the linkage between IVs and DV. However, the findings seem not linking each...

30 August 2018 129 13 View

Doubt on microbiological testing ?

I am conducting microbiological testing on QC samples recently. I did a test to get Total bacterial count (TBC) on a sample. And I used pour plate method. I am encountering some errors recently....

15 August 2018 6,673 9 View

Why Factorial ANOVA table called "Test of between Subject Effects" shows no value (-) in F ratio column for interaction between 3 IVs?

I have run Factorial ANOVA to identify main effect and interaction effect between 3 IVs and 1 DV. The output table called "Test of between Subject Effects" shows no value (-) in F ratio column for...

11 August 2018 7,900 3 View

How can I prepare pure NiO oxide from Ni2O3 black nickle oxide??

Is there any simple heat treatment steps? or should I follow any chemical methods?

05 December 2017 2,453 4 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No title

23 February 2021 4,014 3 View

What is the nutrient requirements for E.coli BL21 CodonPlu RIPL strain growing in M9 medium?

Hello. I want to isotopically label the my protein, and I express it in BL21 RIPL using M9 minimal media. It grows extremely slow. I inoculate the colony from a fresh M9 plate into 5ml M9 medium...

17 February 2021 2,852 2 View

How to measure 50uL of cells?

Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...

14 February 2021 6,247 3 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View

Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

08 February 2021 733 6 View

Does viral RNA extraction from nasopharyngeal swabs require pre-centrifugation with supernatant collection?

06 February 2021 323 3 View

Can I modify vermiculite or perlite by adding nutrients to control a certain situation?

I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...

04 February 2021 8,616 11 View

How to Remove DNA From a Sample Without Rupturing Yeast?

I transformed a plasmid into yeast and selected for transformants by growing them in a selective media (liquid, not agar plate). My experiment requires that I continually grow/ split these...

01 February 2021 9,936 2 View