I have grown a mixed culture of E.coli and lab (different strains) together in a microplate. I would now like to see if lab is alive and would like to enumerate on an agar plate which could only allow lab to grow inhibiting other bacteria from growing? Is it possible? Do we have any specific media for that. I tried growing E.coli on MRS and the E.coli grew pretty well, so I am sure MRS won't work, is there any other culture media which can only allow lactic acid bacteria to grow suppressing E.coli.
Kindly search the literature to see if your bacteria (lactic acid bacteria, according to your question) can grow on phenylethyl alcohol agar. Gram negative bacteria (as E coli) typically do not grow on such medium, but gram positive do.
You can also search for media containing colistin and nalidixic acid. This also prevents the growth of gram-negative spp.
E. coli strains (even toxigenic strains) do not grow in MRS agar. I suggest you to look at the literatures about chromogenic agars. You can easily modify a chromogenic agar such as aniline blue or bromophenol blue agar. Although some research say that lowering pH and lowering sugar in MRS agar would help to grow gram--negative bacteria, there will be no consistency. Also, lactic acid bacteria genus and strains are important in this situation. E.g. L. acidophilus strains grow better in MRS agar than pedococcus spp.
Hi, why don't you try this method!
Article A Rapid and Efficient Screening Method for Antibacterial Com...
You can use the same media you used on microplates.
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