How do we differentiate seed borne endophyte and a pathogen?

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Hi, I would like to test different disinfectants and its effects on biofilms in microplates, which paper has the best protocols!?

I need to do it only in microplates and read the OD value for the results. I cannot use any other assays except microplate. Please help me with this

07 December 2020 5,698 3 View

What are the parameters to assess the effectiveness of online teaching?

Assessment of online teaching

22 October 2020 2,897 23 View

What is the acceptable margin error?

The calculated sample size for my study is 324 with 5% margin error. However, only 230 respondents responded the questionnaire completely. In this case, what is the margin error for this actual...

07 July 2020 524 7 View

What is another alternative to Polyvar 2 Microscope with hot plates and polarizer to check for solubility of drug in lipids?

What is another alternative to Polyvar 2 Microscope with hot plates and polarizer to check for solubility of drug in lipids? To also obtain maximum solubility reading?

02 February 2020 7,999 2 View

What is another alternative to Turbisan Lab Expert to check for nano lipid particles stability?

What is another alternative to Turbisan Lab Expert to check for nano lipid carrier's stability?

02 February 2020 9,150 2 View

What are the essential areas in life long learning?

Areas in life long learning

31 December 2019 9,934 5 View

I am interested to work in Image processing with automation application, looking for tools and resources if any?

Image processing with automation application

11 August 2019 1,945 3 View

XPS spectrum of CuO ?

Why the XPS spectrum of CuO doped in WO3 thin film samples show BE peaks of Cu(0) and/or Cu(I) state of copper and also Cu(II) state of copper. What is the reaction mechanism? Here the CuO doped...

26 July 2019 7,634 8 View

Why TDDFT calculation does not match with the experimental UV-Vis spectrum?

I have calculated the structure of a metal(Ag) complex for which the crystal structure data is available with us. The UV-vis spectrum of the complex shows a very strong absorption at ~250 nm and a...

08 October 2018 221 3 View

What is the difference between Factorial ANOVA and Multiple Regression?

I used same variables (3 IVs and 1 DV) to run factorial ANOVA and multiple regression. I expect the similar findings on the linkage between IVs and DV. However, the findings seem not linking each...

30 August 2018 129 13 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Is it possible to use maize mature seed to do transformation for maize transgenic study?

Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...

17 February 2021 1,434 13 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View

How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

02 February 2021 4,785 3 View