Hi, I would like to test different disinfectants and its effects on biofilms in microplates, which paper has the best protocols!?

I need to do it only in microplates and read the OD value for the results. I cannot use any other assays except microplate. Please help me with this

07 December 2020 5,698 3 View

What is the acceptable margin error?

The calculated sample size for my study is 324 with 5% margin error. However, only 230 respondents responded the questionnaire completely. In this case, what is the margin error for this actual...

07 July 2020 524 7 View

What is another alternative to Polyvar 2 Microscope with hot plates and polarizer to check for solubility of drug in lipids?

What is another alternative to Polyvar 2 Microscope with hot plates and polarizer to check for solubility of drug in lipids? To also obtain maximum solubility reading?

02 February 2020 7,999 2 View

What is another alternative to Turbisan Lab Expert to check for nano lipid particles stability?

What is another alternative to Turbisan Lab Expert to check for nano lipid carrier's stability?

02 February 2020 9,150 2 View

XPS spectrum of CuO ?

Why the XPS spectrum of CuO doped in WO3 thin film samples show BE peaks of Cu(0) and/or Cu(I) state of copper and also Cu(II) state of copper. What is the reaction mechanism? Here the CuO doped...

26 July 2019 7,634 8 View

Why TDDFT calculation does not match with the experimental UV-Vis spectrum?

I have calculated the structure of a metal(Ag) complex for which the crystal structure data is available with us. The UV-vis spectrum of the complex shows a very strong absorption at ~250 nm and a...

08 October 2018 221 3 View

What is the difference between Factorial ANOVA and Multiple Regression?

I used same variables (3 IVs and 1 DV) to run factorial ANOVA and multiple regression. I expect the similar findings on the linkage between IVs and DV. However, the findings seem not linking each...

30 August 2018 129 13 View

Doubt on microbiological testing ?

I am conducting microbiological testing on QC samples recently. I did a test to get Total bacterial count (TBC) on a sample. And I used pour plate method. I am encountering some errors recently....

15 August 2018 6,673 9 View

Why Factorial ANOVA table called "Test of between Subject Effects" shows no value (-) in F ratio column for interaction between 3 IVs?

I have run Factorial ANOVA to identify main effect and interaction effect between 3 IVs and 1 DV. The output table called "Test of between Subject Effects" shows no value (-) in F ratio column for...

11 August 2018 7,900 3 View

How can I prepare pure NiO oxide from Ni2O3 black nickle oxide??

Is there any simple heat treatment steps? or should I follow any chemical methods?

05 December 2017 2,453 4 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

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23 February 2021 4,014 3 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

Peptide precipitation as pre-step for acid hydrolysis for amino acid analysis?

I'm currently experiencing problems during acid hydrolysis for total amino acid analysis as my first samples contain high amounts of sugar (I get too low results back). I therefore had the idea to...

14 February 2021 6,049 3 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

08 February 2021 733 6 View

What is the optimal propagation regime for pET-1M(KanR) and pMCSG7(AmpR) plasmids in DH5alpha E.coli?

Dear all, I am trying to harvest the low-expression plasmids in the mentioned E.coli strain. I constantly gel very low concentrations (30-50 ng/uL while the concentration is usually around 100...

08 February 2021 4,255 4 View