Is Coefficient of Variability (CV) positively correlated with root mean squared error (RMSE) in every case??

My data for fungicide treated replication shows higher RMSE and R-square but less CV compared to the untreated replication for a regression between vegetation indices and yield. How correct is it?...

23 November 2020 2,079 6 View

Where can I get CDS location for COVID-19 sequences of NCBI carona virus database?

Hi All, I am working on developing a computational model to find protein coding and non-coding regions of COVID-19 DNA sequences. To test my model, I need CDS information of COVID-19 DNA...

30 April 2020 4,162 7 View

How to calculate sensitivity and specificity using Genscan Tool?

Hi, Can anyone please explain me how to use Genscan tool to find exons and introns for a given gene sequence? I want to calculate sensitivity and specificity to check its performance for a given...

30 December 2019 2,083 3 View

Is it necessary to have a reference electrode for EIS if the counter electrode is sufficiently large?

I am studying water splitting on Pt substrates. Is it necessary to use a reference electrode or is it possible to study the system using a two electrode setup where the Pt counter electrode is...

19 November 2019 7,182 3 View

Scalogram for huge data?

I have a huge data for which I want to apply wavelet and plot scalogram. the below code works for small data. However, I have a huge signal, for which I am not able to plot scalogram. Can anyone...

10 April 2019 1,959 3 View

May I know the difference between absolute reflectance and reflectance(transmittance) in ASD based measurements?

I am trying to process the wheat crop data collected using ASD for reflectance. However, I see two different options in ViewSpecPro software- Absolute reflectance and reflectance(transmittance)....

25 March 2019 1,488 4 View

How do I find out the flanking ends or the complete genome sequence of a given sequence that I have obtained from a PCR product?

The organism is Streptomyces but the exact strain is not known and hence the sequence. So after sending the PCR product for sequencing we have the part of the whole sequence that contains the gene...

26 February 2019 3,182 4 View

How to statistically quantify sorghum grain yield loss by birds?

I had 15 genotypes of sorghum planted in 6 replications. There was pre-harvest grain yield loss by birds that made it difficult to compare these genotypes based on yield performance. Is there any...

20 December 2018 2,294 3 View

Why there is no nitrogen in the reactions in Eddy Dissipation or Finite Rate/Eddy Dissipation?

I don't see nitrogen in the oxidizer(air). Should I consider nitrogen while calculating mass fractions?

08 September 2018 7,522 1 View

How to calculate the mass fractions of species in multi-step reactions?

I'm trying to simulate premixed combustion in fluent. I'm using methane-air mixture for combustion with a 2 step reduced mechanism. I'm confused about the mass fraction calculations.

01 September 2018 8,371 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View