For gels/ointment preparation is there any natural alternatives for CMC?
What functionality do you want to replace?
If it's just a thickener, there is xanthan. For a polymeric emulsifier there is gum Arabic...
Use a substitute like xanthan gum, guar gum or pectin as a thickening agent.
Is it necessary to run the Rt PCR product on agarose gel after completion?
29 December 2020 4,823 3 View
I have downloaded the MSWEP sub-daily rainfall data (3-hourly data 0.1 degree resolution). I used MATLAB to convert it and obtained a table of dimensions 1800 x 3600 for a specific date. I am...
18 October 2020 4,205 4 View
I am trying to model a jet fan being installed in a room. I want to study the flow of the fan coming out of the outlet (basically the development of streamlines). The walls of the room are...
12 October 2020 4,970 3 View
I am new to abaqus and I am trying to optimize a shape of a segment of a model (As a part of my master thesis). Please refer to the example model I have attached. I need to optimize the segment...
23 August 2020 5,828 9 View
I have submitted one of my review article on COVID 19 and ophthalmology in April through online submission but journal haven't overlooked it inspite of several request. Even i have sent request...
06 August 2020 1,802 3 View
Hi I am trying to find some information as how to link up OpenFoam and Star CCM, however I am not able to find adequate information. Below is the details of the project. 1- Objective: to...
05 June 2020 8,484 3 View
I am using python 3.6.8 on a Windows 10 machine. We can only move to subdirectories from the default installation (root) folder in jupyter. I wanted to start the notebook from a particular...
08 March 2020 9,259 4 View
HEMT FABRICATION.
24 February 2020 4,958 3 View
Hello, I am new to surrogate-based data analysis. In my model, Input space consists 6 variables and a single ouput is noted from each simulation. I have N such simulations. ( Sampling method is...
09 February 2020 7,511 3 View
I am investigating the mechanical and physical property changes in the seawater aged GFRP. I am requiring a suggestion of to what maximum number of days should I age it. Is there a particular time...
30 January 2020 570 4 View
I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...
27 February 2021 9,176 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...
24 February 2021 6,310 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...
23 February 2021 8,249 2 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View
Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...
22 February 2021 9,896 3 View
I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...
18 February 2021 6,736 4 View
I need to work with free lipids at increasing concentration (50%, 60% of the CMC ect.). I'm now working with POPC and POPG. To reach these concentrations I have to start from concentration over...
17 February 2021 1,676 3 View