What is the natural alternative of CMC in cosmetics?

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How to carryout antibacterial activity for a material such as complex nanomaterial that is insoluble and forms suspension only?

I need to carry out antibacterial activity testing by disc diffusion or agar well diffusion followed by MIC by agar microdilution. The sample I have is a nanocomposit which doesn't dissolve in any...

16 February 2024 7,046 2 View

Having difficulty with relaxing my system using Q.E?

Hi all, I am having difficulty with relaxing my system using Q.E. I've tried several times but calculation just stops after some time duration. Last line of output file gives me "Davidson...

04 February 2024 5,788 0 View

Is it necessary to check if our data is normal before carrying out statistical analysis such as t test and ANOVA?

One of the many assumptions in ANOVA is that our data follows a normal distribution. Usually in biology experiments are carried out in triplicates and the avalilable data is very small, not >...

28 January 2024 6,579 8 View

Can anyone provide me the structure (CIF file) of Berlin Green .Structural Formula is Fe[Fe(CN)6]?

I need CIF file of Berlin Green.

21 January 2024 6,340 1 View

Is there any host plant to separate CMV and potyvirus?

I have plant sample with mixed infection of CMV and potyvirus, is there any way I can separate CMV only?

10 January 2024 3,972 1 View

I want to culture and expand MSCs in serum free condition to isolate small EVs. What is the alternative to FBS If I have DMEM KO as basal media??

I'd prefer not buying any separate commercial media because of the cost. Please suggest what growth factors or vitamins can I add to DMEM KO other than glutamax, penstrep and bFGF for the MSCs to...

08 January 2024 6,443 3 View

Seeking Guidance on Variant Analysis in Differentially Regulated Genes using RNA-Seq Data?

I am analyzing RNA-Seq data, specifically focusing on the identification of variants within differentially regulated genes across treated and untreated samples. My primary analysis platform for...

01 January 2024 4,716 0 View

Remedies in civil law?

Civil remedies

01 January 2024 3,466 3 View

Any suggestions on obtaining epitope information for a specific splice variant of gene X from Cell Signaling or Abcam antibodies?

I am currently in need of an antibody targeting gene X, specifically focusing on a distinct splice variant where the major variance lies within the N-terminal region. Unfortunately, suppliers like...

26 December 2023 8,468 2 View

How to find the information about a particular transcript variant using a genome browser?

What tools or methodologies are available to comprehensively analyze RNA expression profiles across various human tissues, specifically to discern and compare transcript variants of a gene

19 December 2023 7,167 7 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Why is my ASO degraded quickly on agarose gel?

I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...

06 August 2024 3,130 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to check pH of a high strength hydrogel?

We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...

30 July 2024 942 2 View

Following click reaction in cell lysates, protein is immobile and remains at the top of the gel in SDS-PAGE?

I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...

29 July 2024 950 4 View

Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What is the cause of this aggregation just bellow 70 kDa mark?

Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...

21 July 2024 5,128 4 View

Why my gel electrophoresis have shadow bands? Please see the attached picture for the gel electrophoresis ?

Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...

19 July 2024 148 6 View

Can anyone help in getting software related to gel scoring of silver stained PAGE for diversity study?

I have conducted molecular marker amplification experiment by PCR and performed PAGE with silver staining. Now i have to score all my gels for further genetic analysis. Manual gel reading is...

12 July 2024 7,145 2 View