For gels/ointment preparation is there any natural alternatives for CMC?
What functionality do you want to replace?
If it's just a thickener, there is xanthan. For a polymeric emulsifier there is gum Arabic...
Use a substitute like xanthan gum, guar gum or pectin as a thickening agent.
I need to carry out antibacterial activity testing by disc diffusion or agar well diffusion followed by MIC by agar microdilution. The sample I have is a nanocomposit which doesn't dissolve in any...
16 February 2024 7,046 2 View
Hi all, I am having difficulty with relaxing my system using Q.E. I've tried several times but calculation just stops after some time duration. Last line of output file gives me "Davidson...
04 February 2024 5,788 0 View
One of the many assumptions in ANOVA is that our data follows a normal distribution. Usually in biology experiments are carried out in triplicates and the avalilable data is very small, not >...
28 January 2024 6,579 8 View
I need CIF file of Berlin Green.
21 January 2024 6,340 1 View
I have plant sample with mixed infection of CMV and potyvirus, is there any way I can separate CMV only?
10 January 2024 3,972 1 View
I'd prefer not buying any separate commercial media because of the cost. Please suggest what growth factors or vitamins can I add to DMEM KO other than glutamax, penstrep and bFGF for the MSCs to...
08 January 2024 6,443 3 View
I am analyzing RNA-Seq data, specifically focusing on the identification of variants within differentially regulated genes across treated and untreated samples. My primary analysis platform for...
01 January 2024 4,716 0 View
Civil remedies
01 January 2024 3,466 3 View
I am currently in need of an antibody targeting gene X, specifically focusing on a distinct splice variant where the major variance lies within the N-terminal region. Unfortunately, suppliers like...
26 December 2023 8,468 2 View
What tools or methodologies are available to comprehensively analyze RNA expression profiles across various human tissues, specifically to discern and compare transcript variants of a gene
19 December 2023 7,167 7 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
I am using CuBr/THPTA for a click reaction in total cell lysates. I am facing issues with my protein sample in non-reducing SDS-PAGE where it's not migrating properly and most of it remains at the...
29 July 2024 950 4 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
Hello, I was running a 12% SDS Page electrophoresis on few granulosa cell samples and got this result after the ponceau staining. The total protein lysate seem to aggregate at 70 kDa ladder mark...
21 July 2024 5,128 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
I have conducted molecular marker amplification experiment by PCR and performed PAGE with silver staining. Now i have to score all my gels for further genetic analysis. Manual gel reading is...
12 July 2024 7,145 2 View