What is the natural alternative of CMC in cosmetics?

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For Rt PCR?

Is it necessary to run the Rt PCR product on agarose gel after completion?

29 December 2020 4,823 3 View

How to interpret MSWEP 3-hourly rainfall data 0.1 degree resolution?

I have downloaded the MSWEP sub-daily rainfall data (3-hourly data 0.1 degree resolution). I used MATLAB to convert it and obtained a table of dimensions 1800 x 3600 for a specific date. I am...

18 October 2020 4,205 4 View

Fan boundary condition using Ansys Fluent?

I am trying to model a jet fan being installed in a room. I want to study the flow of the fan coming out of the outlet (basically the development of streamlines). The walls of the room are...

12 October 2020 4,970 3 View

In Abaqus- How to optimize the shape of a particular region while keeping displacement at two points equal?

I am new to abaqus and I am trying to optimize a shape of a segment of a model (As a part of my master thesis). Please refer to the example model I have attached. I need to optimize the segment...

23 August 2020 5,828 9 View

Shall I submit the article simultaneously to another journal even after request to first journal to withdraw the manuscript???

I have submitted one of my review article on COVID 19 and ophthalmology in April through online submission but journal haven't overlooked it inspite of several request. Even i have sent request...

06 August 2020 1,802 3 View

How do you transfer files from Open Foam to Star CCM ?

Hi I am trying to find some information as how to link up OpenFoam and Star CCM, however I am not able to find adequate information. Below is the details of the project. 1- Objective: to...

05 June 2020 8,484 3 View

How to start jupyter notebook from a particular folder instead of default root folder?

I am using python 3.6.8 on a Windows 10 machine. We can only move to subdirectories from the default installation (root) folder in jupyter. I wanted to start the notebook from a particular...

08 March 2020 9,259 4 View

Is it OK to anneal GaN HEMT epitax after some AlGaN recess. what are its pros and cons?

HEMT FABRICATION.

24 February 2020 4,958 3 View

Best resources for Multivariate Kriging for deterministic simulation with Python ?

Hello, I am new to surrogate-based data analysis. In my model, Input space consists 6 variables and a single ouput is noted from each simulation. I have N such simulations. ( Sampling method is...

09 February 2020 7,511 3 View

What might be the maximum time of immersion required for the sea water ageing of glass fiber reinforced polyester composites?

I am investigating the mechanical and physical property changes in the seawater aged GFRP. I am requiring a suggestion of to what maximum number of days should I age it. Is there a particular time...

30 January 2020 570 4 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

Sanger Sequencing results not matching experimental results of Restriction Digestion, colony PCR and protein expression?

I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...

18 February 2021 6,736 4 View

How to obtain free lipids starting from concentration over the CMC?

I need to work with free lipids at increasing concentration (50%, 60% of the CMC ect.). I'm now working with POPC and POPG. To reach these concentrations I have to start from concentration over...

17 February 2021 1,676 3 View