How do I go about with a Dendritic cell-T cell co-culture experiment?

30 November 2022 0 2K Report

I want to detect the T cell differential when it co-culture with dendritic cells with the presence of OVA

In some research, immature BMDC co-culture anti-CD3、anti-CD28 stimulated naive CD4+T cell from wild type mice, and most of research use OT-II naive CD4+T cell, why OT-II naive CD4+T cell do not need stimulation of anti-CD3、anti-CD28 ?

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