I grow cells in defined 3D hydrogels and the cells get impregnated within. I want to get a good yield of the cells for protein extraction. Has anyone worked with cell extraction from gelatin hydrogels?
You may use low concentration of collagenase with mild agitation for some times in 17 degreeC. It will followed by the centrifugation for 2-3 minutes. You may try and best of luck!