How do I etch In2O3/Au/Ag coating from an optical grade polyester (PET) film?

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How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

Why do these abnormal charge/discharge curves of coin cell batteries appear?

As shown in the figures, there are several abnormal charge/discharge curves.Each of them may have different problems. I am curious why these situations appeared differently?There are a lot of...

24 July 2024 6,930 1 View

How to troubleshoot the Milliplex Cytokine assay, when I see >OOR/<OOR in my data?

I am conducting cytokine measurements using a multiplex assay. Some of the proteins are yielding results that fall outside the standard curve values, either below (< OOR) or above (> OOR)....

24 June 2024 9,717 0 View

How can formulate Phosphorus 52 and Potassium 34 in liquid ?

formulate Phosphorus 52 and Potassium 34 in liquid

03 June 2024 7,589 2 View

"Range chek error" problem in DSSAT Model?

I was previously using the DSSAT version 4.7 in my PC. Now, I have installed the DSSAT v4.8. Whenever I am trying to simulate the experient is shows "Range chek error". This problem exist even...

13 May 2024 5,894 0 View

I am trying to do X ray peak broadening analysis of nanosheets. How important it is to strip the K-alpha 2 for accurate results?

The stress-strain calculations, energy density GI index calculations are also being done.

02 May 2024 9,963 4 View

How have solitons been interpreted in the protein folding mechanism?

One and two soliton solutions have been obtained while considering the protein folding mechanism of Davydov solitons. What are the dynamics of these soliton solutions that can be obtained in...

25 April 2024 5,241 0 View

Has anyone designed guideRNA for multiple targets using polycistronic-tRNA-gRNA expression system?

I am planning to design guideRNA for multiple targets to express as a polycistronic-tRNA-gRNA expression. I would like to know whether we need to include any linker between the tRNA and guideRNA...

29 March 2024 1,055 0 View

In RG 80 Nobel Prize Winners are listed ,Can we know their area of research and institute of affiliation with country ?

The answer help young researchers get motivated

19 March 2024 3,204 1 View

How to make 10% Boron solution , which content 10% elemental Boron value ?

Procedure for the preparation of 10% Boron liquid.

20 December 2023 9,622 2 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

How to define an anisotropic material with asymmetric elastic compliance/stiffness matrix in ANSYS APDL?

I need to model an anisotropic material in which the Poisson's ratio ν_12 ≠ ν_21 and so on. Therefore, the elastic compliance matrix wouldn't be a symmetric one. In ANSYS APDL, for TB,ANEL...

09 August 2024 5,048 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Stability of the Solar System: Insights from Einstein’s Equations ?

The stability of the Solar System is a complex subject that blends the classical framework of Newtonian mechanics with the modern insights provided by General Relativity (GR). Understanding this...

07 August 2024 2,569 1 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How can I measure the thickness of thin film by Uv-Vis?

06 August 2024 7,810 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View