Am working with the bacteria Ralstonia solanacearum and would like to prepare inoculum concentrations lower that the level known to cause disease i.e 106 CFU/ml and 104 CFU/ml while using the spectrophotometer to determine OD600nm. Is this achievable? How then do I convert OD measurements to CFU/ml?
This should help you :
https://www.researchgate.net/post/What_is_standard_procedure_for_making_bacteria_standard_curve_using_OD_and_CFU_mL
There are several methods, but the general idea is that the relationship between CFU/ml and OD600 will be linear. It is especially true if you plan to dilute your bacterial suspension after growth (to inoculate plant materials for example). You just have to make serial dilutions of your bacterial suspension (1/10, 1/100, 1/1000 or 1/2, 1/4, 1/8, 1/16) and to measure OD600. Then, you just have to plate 100 µl on 3-5 agar plate for each dilutions and count the CFU after incubation. Obviously, only some dilutions will be countable.
Regards,
Thank you very much Adrien Biessy .
Your answer will be of great help in my studies.
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