How do I confirm that my suspension cells are been infected after transduction?

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What are theories behind cash reserve ratio and monetary policy rate?

The reason for the question is because those monetary policy instruments originated from somewhere.

18 April 2024 764 2 View

In studying the leadership style of a university, whose leadership style is the researcher supposed to measure - the VC or who?

I am studying the impact of leadership style on the performance of a university. Whose leadership style am I supposed to study?

10 March 2024 5,426 6 View

Could someone kindly recommend a suitable time-lapse camera for use under a microscope?

Greetings, I am in search of a time-lapse camera suitable for use under a microscope. Could someone kindly provide recommendations for a suitable model and suggest where it can be purchased? Thank...

02 March 2024 9,676 0 View

Respectful greetings to all. How to control and guide urbanization in developing countries?

I'm trying to embark on a new research topic, I would like to have your opinions and suggestions

17 December 2023 5,491 4 View

Can project management techniques be used in a scientific research project?

Project management takes into account initiation, planning, execution, evaluation of milestones and closure. Is it possible to take it into account in a scientific research project?

05 December 2023 7,027 0 View

How can we apply techniques to mitigate the effects of climate change due to greenhouse gases and deforestation in developing countries?

Reducing emissions from deforestation and forest degradation in developing countries is a problem that challenges many environmental organizations. A contribution on this question would be a step...

23 November 2023 1,880 4 View

What software to use to make geochemical diagrams for advanced research?

To give more chance to my next research, I would like to use advanced graphs and diagrams for geochemical data processing

22 November 2023 3,367 2 View

Hey there. How can I visualize a past volcanic eruption using remote sensing?

I'm trying to set a new subject

14 November 2023 1,965 3 View

Hi there. Can anyone recommend some papers on concrete compression testing and sand analysis?

I'm working on a new paper with my research team. The aim is to find the best type of sand by comparing their geotechnical characteristics

12 November 2023 8,287 0 View

Hey all. How to assess the flow of a volcano during an eruption?

I'm trying to work on a new topic with a new challenging subject

12 November 2023 4,120 0 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

Can I Use D-PBS for Preparing a 10% Sucrose Solution for Lentivirus Concentration?

Hello, I am a master's student currently conducting lentivirus transduction experiments in Jurkat cells. The virus is not effectively entering the Jurkat cells, so I am planning to use an...

21 July 2024 4,676 4 View

What will be the best way to test NFkb activation via western blot?

Hi, I have lysate from human macrophages, and I wonder what will be the best way to check the NFKB activation in those cells. I was thinking p-p65 or p-ikkb./ Is that make sense?

18 July 2024 5,214 4 View

Lentivirus transduction of HEK293T cells?

i need to ask that when I transduce my HEK293T cells with Lentivirus, how much time will they require for transduction because when I add lentivirus and then change the media after 24 hours, my...

07 July 2024 2,946 1 View

Why the fibroblasts transfected with puromycin plasmids are dying after treatment of puromycin?

Hello, everyone. I would like ask one question. I transfected fibroblasts with EF1A-myod-puro using lipofectamine 3000. Promoter for puro is CMV. After transfection 48 hours, I did puromycin...

23 June 2024 4,902 3 View

Can anyone send me the detailed protocol of lentiviral titer determination and its invitro transduction into Hela cell line or MSCs?

I have searched a lot of information from the published article but couldn't find valuable. As I have prepared the Lentiviral vector and stored it in -80 degree!

21 June 2024 3,484 1 View

How do I successfully culture primary human leukocyte from TLRE filters and improve viability of the cells in suspension?

I am currently culturing leukocytes from TLRE filters from our blood bank. I have been struggling to culture these cells, as well as improve their viability. I was wondering if anyone would be...

16 June 2024 3,752 0 View

Is it possible to customize the acoustic lens of the ultrasonic transducer in Field II?

We are interested in studying the effects of varying lens curvatures and material properties on the acoustic field. If this is not feasible, could you recommend alternative software that allows...

15 June 2024 6,213 0 View

How to adjust or recalibrate negative MFI values from cytometer?

I am looking at puromycin incorporation rate by flow cytometry using a specific anti-puro-antibody which I use to stain different cell types. For some cells the signal is high (i.e. Puro MFI),...

12 June 2024 1,110 1 View