How do I confirm that my suspension cells are been infected after transduction?

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Can anyone help me with an instrument or a questionnaire to measure INNOVATION Culture of SMEs?

I am conducting a research and one of my variables is INNOVATION CULTURE. Please, can someone assist me with an instrument or a questionnaire to measure the innovation culture of SMEs?

04 September 2020 4,892 8 View

What the diferencence between municipal and public international law?

the definations and diferences between the 2 laws

24 February 2020 1,992 3 View

Is soxhlet extraction a reliable way of getting pure THC from cannabis plant?

I have been having isssues getting pure THC form the USA and it seems the price from sigma is exhorbitant. I want to get the plant and extract THC. i was told by someone that soxhlet extraction...

15 August 2018 8,300 9 View

Can antibodies be produced against an antigen that has a molecular weight of 318.49 while conjugated with Freunds adjuvant and given booster inj.?

I want to challenge rabbits with an antigen having a molecular weight of 318.49 to harvest antibodies. is it possible for antibodies to be produced against this antigen after mixing it with...

09 November 2017 2,324 3 View

Can someone help with the ideal procedure for embryonic GIT histochemistry and immunohistochemistry?

02 February 2017 2,226 3 View

Besides use of ethanol, is there any other alternative medium to which fecal samples can be stored before transport to the laboratory for analysis?

Besides use of ethanol, is there any other alternative medium to which fecal samples can be stored before transport to the laboratory for analysis? transport medium for fecal samples for stability...

16 July 2016 9,071 1 View

I would like to know about the method of separating chlorophyll from ethanolic extracts from celtis africana?

The techniques of separation of chlorophyll from methanolic plant extracts.

20 May 2015 8,064 4 View

Which is the best finite element software for modelling the effects of temperature and pressure on a oil well cement sheath?

This for a MSc Project.

30 November 2014 7,379 2 View

Coomunication strategies for behaviour change

If i want to market a health product what is the best way to approach a semirural market about the services I offer. back your answers with a simple reason for your answer choice

01 January 1970 3,553 4 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Is RD-ES grow attached or in suspension or both?

We have a old RD-ES cell line which is grow attached, but we got another vial of RD-ES which is very easy to get detached and grow in sphere in the medium. Do you know which morphology is normal...

24 February 2021 5,183 1 View

Why is there no protein expression but antibiotic resistance in transduced A549 cells with pLVX-TetOne-Puro lentivirus?

Hi, I have been trying to transduce A549 and Calu-3 cells using the Lenti-X Tet-One inducible expression system (TakaraBio). My gene of interest is inserted in a pLVX-TetOne-Puro plasmid and has...

15 February 2021 8,462 3 View

Is there a way in which microglia can be extracted from mice without killing them ?

I'm aiming to use an adoptive cell transfer approach, with microglia in mice with ALS. So far all the protocols I have seen, require sacrificing the mouse. I'd like to re-infuse the microglia back...

11 February 2021 9,509 1 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View

Calculating the CFU?

i am constructing research for the antibacterial activity of the medication and I am testing it by %RCC, so my Q after I did 10 serial dilutions for the bacterial suspension can we do plating for...

04 February 2021 5,886 1 View

How accurate is the shear modulus measured using PROCEQ DPC transducer of 40kHZ frequency on 100mm concrete cube specimen?

It is required to evaluate shear modulus of concrete cube specimen of 100mm size. Since the diameter of DPC transducer is around 84mm, I would like to know whether it can give reliable shear wave...

02 February 2021 1,052 3 View

Does gene insertion via CRISPR/Cas9 result in chromosomal abnormalities?

Hello, I hope to insert a gene into mammalian cells in culture using CRISPR/Cas9 and an HDR template. Will insertion of a 1 kilobase gene cause chromosomal abnormalities and effect cell...

31 January 2021 2,516 1 View

Why is my GOI not expressing after lentiviral transduction despite RNA increase and protein is detected in transfection?

Hi, I'm trying to create a stable overexpression line with EF1a-FlagGOI-IRES-GFP using lentivirus packaged with pxPAX2 and pM2G. After transduction of 293T with 8ug/ml polybrene, I was able to...

29 January 2021 1,878 4 View