Innocent Agida

5 Questions 6 Answers 0 Followers

Questions related from Innocent Agida

How do I perform MOI ?

Good day everyone, Please I am doing transfection/ transduction, I want to check the MOI of the virus and at the same time perform viral concentration, but i am stuck up with the steps. please can...

22 July 2023 861 2 View

Good day everyone, I added puromycin to my cells, How do I select positive clones?

I am trying to knockout a gene from my cells, I am stuck after I have infected my cells with the desired lentivirus construct and I have added puromycin. Please I need help on how to go about the...

08 July 2023 1,519 3 View

Good day everyone! Please I want to do transfection and Lentivirus packaging, and also lentivirus infection on my cell lines 293FT cells, how do it?

Good day everyone! Please I want to do transfection and Lentivirus packaging, and also lentivirus infection on my cell lines 293FT cells, how do it. Please i need a step by step guide, I will be...

23 June 2023 9,821 2 View

Hello everyone. Please i will be performing CRISPR cas 9, I dont know how to do this, i need help. who can assist me please ?

Good day everyone, Please I will be performing CRISPR cas9, I don't know how to go about this, I will appreciate it if anyone can assist me, I don't mind having a video to guide me step by step....

07 June 2023 2,877 3 View

What volume of medium is required to be added to 100mM stock of Doxorubucin to have different concentration of 0.1, 0.5, 1, 1.5, 2, 2.5, 5 and 10uM ?

Hello everyone, Please I am trying to prepare different concentration of doxorubicin for my cancer cells line, Can someone help me with the calculations so I can continue my experiment. Thanks in...

19 May 2023 697 2 View