Innocent Agida

13 Questions 10 Answers 0 Followers

Questions related from Innocent Agida

What is the optimum Plasmid concentration after extraction?

Hello everyone, please what is the optimum Plasmid concentration after extraction with the mini prep method, Please your kind response will be helpful. Thanks !

13 November 2023 2,261 3 View

How to transfeect suspension cells, DB and Karpas-422 for insatance to have a maximum transfection efficiency?

Hello everyone, please I have been having difficulties in transfecting DB and Karpas-422 suspension cells with the lentivirus, can anyone suggest or advise me on the possible solution, I have...

13 October 2023 7,633 0 View

How do I select a single clone from a population of positive cells after puromycin selection ?

Good day everyone, Please how do I select a single clone, from a population of positive cells, I have done puromycin selection and now I want just a single clone from the cell population but I am...

09 October 2023 6,978 3 View

How do I confirm that my suspension cells are been infected after transduction?

Hello everyone, please I am trying to transduce my suspension cells, I don't know if the transduction is successful so as to continue with the puromycin selection, please I will appreciate your...

23 September 2023 8,996 2 View

What could be the reasons why some proteins are expressed afer a knockdown seen after performing WB and none was transcribed after a qPCR ?

I did a knockdown, after checking the level of protein expression with Western blot, I got some percentage let's say like 60 to 70 % of knockdown, but when i tried checking with qPCR to check the...

30 August 2023 726 3 View

How do I perform MOI ?

Good day everyone, Please I am doing transfection/ transduction, I want to check the MOI of the virus and at the same time perform viral concentration, but i am stuck up with the steps. please can...

22 July 2023 897 2 View

Hello everyone ,I am trying to make a killer curve with puromycin please how do I prepare different concentration of puromycin for killer curve ?

Hello everyone, Please I am trying to prepare a killer curve for my cell lines with puromycin, I need different concentration for the puromycin to be used, I am stocked on how to get this done,...

10 July 2023 1,264 1 View

Good day everyone, I added puromycin to my cells, How do I select positive clones?

I am trying to knockout a gene from my cells, I am stuck after I have infected my cells with the desired lentivirus construct and I have added puromycin. Please I need help on how to go about the...

08 July 2023 1,524 3 View

Good day everyone! Please I want to do transfection and Lentivirus packaging, and also lentivirus infection on my cell lines 293FT cells, how do it?

Good day everyone! Please I want to do transfection and Lentivirus packaging, and also lentivirus infection on my cell lines 293FT cells, how do it. Please i need a step by step guide, I will be...

23 June 2023 9,828 2 View

Hello everyone. Please i will be performing CRISPR cas 9, I dont know how to do this, i need help. who can assist me please ?

Good day everyone, Please I will be performing CRISPR cas9, I don't know how to go about this, I will appreciate it if anyone can assist me, I don't mind having a video to guide me step by step....

07 June 2023 2,884 3 View

Hello everyone, How do I get the exact volume of cell suspension to seed?

Hello everyone, I am currently trying to do a cck-8 assay, Please I need an assistance, I am having trouble to get the exact volume of cell suspension to seed. I need 5000 cells and I have checked...

29 May 2023 3,050 2 View

What could be the reasons for not having a CT value after a qpcr analysis?

I tried doing a qpcr analysis after the whole process I got no Ct value everything, washing negative on the analysis table.

22 May 2023 8,390 1 View

What volume of medium is required to be added to 100mM stock of Doxorubucin to have different concentration of 0.1, 0.5, 1, 1.5, 2, 2.5, 5 and 10uM ?

Hello everyone, Please I am trying to prepare different concentration of doxorubicin for my cancer cells line, Can someone help me with the calculations so I can continue my experiment. Thanks in...

19 May 2023 708 2 View