I am looking at puromycin incorporation rate by flow cytometry using a specific anti-puro-antibody which I use to stain different cell types. For some cells the signal is high (i.e. Puro MFI), while for other cells the MFI is negative!!
Would that be a problem of scaling the values for theis marker or adjusting the voltages of the PMTs on the cytometer?
Thank you for any advice or assistance you may offer.