How to disaggregate country data into city ones?

I'm using a number of indicators in my research which are not available at a sub-national level. Is there a reliable method for disaggregating the country data into city ones? (preferably with a...

16 February 2021 6,861 3 View

Cross-lagged panel analysis and sample size?

I want to estimate the causal relationship between two latent variables over time using structural equation modelling. both latent variables are measured according to different indicators...

31 January 2021 3,777 5 View

IHC-paraffin experiment: how do you approach shopping for an antibody?

Hello everyone! I am shopping for an arginase 1 antibody to use for IHC-paraffin experiments with rat tissue. So far what I have understood is that if an antibody says it is reactive with rat,...

16 January 2021 794 3 View

SEM for panel data ?

I'm trying to find the effect of smart cities on sustainable development. I collected data for several indicators of smartness of a city for 15 cities over 12 years. seems like panel data (GMM...

12 January 2021 9,914 5 View

How to prepare H2O2 solution or certainly how can we prepare 30% w/v H2O2 solution?which solvent?

Inorder to enhance the photocatalytic activity of magnetic nano photocatalysts like zinc ferrite we can use H2O2.

10 November 2019 5,780 3 View

Does anyone know where i can purchase drug-permeable e. coli?

I need an e. coli strain that's membrane is permeable to antibiotics.

10 October 2019 2,177 3 View

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18 May 2019 5,751 2 View

Can anyone suggest me to set up a suitable basis set for theoretical calculations of organometallic complex?

I have recently installed Gaussian 16 for dft calculations. I have no good idea about suitable basis set? How to specify basis set for heavy atom and light atoms? YouTube tutorial mainly related...

16 February 2019 9,237 4 View

What are the tips to be considered for peptide encapsulation in liposome?

I am working on nanoliposome formulation to encapsulate a synthetic peptide , I have tried many ways for encapsulation, but after dialysis there wasn't any peptide in liposomes which I analyzed by...

02 December 2018 8,010 7 View

How do I analyse my luciferase data?

Hi I'm looking for some help in analysing my luciferase data. I have a transcription factor and then a range of proteins I think will influence transcription factor activity. For each...

08 November 2018 4,617 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View