What is the cause of bubbles formation DURING qPCR and how to mitigate it?

I am analyzing residual DNA in samples containing a high concentration of protein, therefore I have to do the extraction protocol. However, my results have been very inconsistent, ranging from...

18 November 2020 3,514 3 View

What is the best mitigation plan for school and community that sited to the river which is contaminated with chemicals released from nearby industry?

The river is classified as a category V contaminated river. Teacher claim that the bad smell often after and during raining.

26 July 2020 1,654 3 View

Is it possible to apply statistics on synthesis and anticancer activity?

I m looking for paper having knowledge of statistics (spss or matlab) applied on synthesis and anticancer activity

20 March 2020 5,517 3 View

Could you anyone share the authentication code of blockchain?

I am working on cooja simulator for the Authentication of iot node with blockchain. could you anyone share the authentication code of blockchain?

30 January 2020 4,452 7 View

How to conduct UV vis spectroscopy of Cr doped ZnO?

We are to conduct UV vis of Cr doped ZnO but we are stuck in making solution... di water doesn't work... can we use HCl as a solvent for such transition metal doped metal oxides.. how about...

05 December 2019 8,397 12 View

How do I dissolve water in oil (w/o) nanoemulsion in water?

I did quick preliminary solubility study on my flavonoid enriched extract in oil and water phase. I found my extract is more soluble in water. Prior this study, I thought my extract is oil soluble...

17 July 2019 5,198 3 View

What are the companies producing active compounds i.e. flavonoids?

With regard to the question above, may I know any good companies are extracting, enriching or isolating natural active compounds i.e.flavonoids for pharmaceutical, nutraceutical or cosmaceutical...

08 May 2019 7,051 1 View

I would like to know about how can crystallization of metal complexes take place?

When i prepare the complexes it forms in amorphous state i want to make crystals of complex.my complexes soluble DMSO but when i dissolved in it and pour insoluble solvent it was oxidized.

15 February 2019 7,053 2 View

What if the absorbance value of plant extract is higher than the range of calibration curve?

I am currently doing research on total flavonoid content. I use Quercetin as standard for my calibration curve. The regression coefficient is 0.99. I use different solvent extracts as my samples....

30 January 2018 9,342 9 View

Why YbF3 nanopowder color becomes gray or black after sintering?

YbF3 powder color becomes gray/black when I sinter at 400C or 500C or 600C even when I sinter with a cover on the powder. I am using Yb acetate as a Yb source. When I use Yb nitrate as a source,...

17 January 2018 4,716 6 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What test should i use in comparing the correlation between categorical and continuous variables?

Dear all, I have the variable (Surgery duration) represented by values in minutes and the variable (preoperative neuropraxia cases) represented as (Yes/No) values. I want to compare these two...

03 March 2021 5,427 5 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

The procedure compute?

02 March 2021 5,204 3 View

Does anyone know what a '.' means in SPSS?

Hi everyone! I was wondering if anyone would be so kind as to help me with regards to my data set on SPSS! I was checking for multivariate outliers via the standardised residuals ('Linear...

02 March 2021 7,910 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View