How can I validate the UV spectroscopy?

can I compare UV spectroscopy reading with nanodrop reading to validate the UV spectroscopy? or is there a better way to know if the UV works well or not? Note: we use it in DNA, RNA &...

12 December 2020 4,869 2 View

What's the right way to Tween 20 storage?

02 December 2020 9,955 2 View

What's makes RNA (and not DNA) bind to the silica spin column in commercial kits?

In silica spin column-based kits, if the spin column material is the same and the binding to the column occurs by using ethanol and Guanidine salts. Then, what's the difference between the RNA...

20 September 2020 4,798 1 View

Is it possible to apply statistics on synthesis and anticancer activity?

I m looking for paper having knowledge of statistics (spss or matlab) applied on synthesis and anticancer activity

20 March 2020 5,517 3 View

Why Non specific bands of PCR reaction appear while using specific primers for tobamoviruses?

i have done RNA extraction and cDNA for tobamovirus then i made a PCR reaction using primer pair Tob uni1/Tob uni2 according to the protocol in Letschert et al., 2002 ( annealing temp. 60C for 45s...

09 December 2019 7,276 3 View

I would like to know about how can crystallization of metal complexes take place?

When i prepare the complexes it forms in amorphous state i want to make crystals of complex.my complexes soluble DMSO but when i dissolved in it and pour insoluble solvent it was oxidized.

15 February 2019 7,053 2 View

Help Please!! how to solve the error "too many attempts made for this increment" in abaqus?

i am applying a load of 50KN gradually on a reinforced concrete beam, i'm unloading this load using an amplitude to get the loading and unloading versus deflection graph.i have used concrete...

09 June 2017 3,129 14 View

Hello How to get a producer to dissolve cellulose fiber by carbon disulfide ?

11 April 2017 9,899 2 View

How to get the frequencies of simply supported RC beam when changing load in ABAQUS?

i have modeled the RC beam with simply supported BC and used concrete damaged plasticity model to account for the nonlinearities in concrete, i have got the frequency value for the case of beam...

01 January 2017 737 6 View

What kind of experiment help in understanding the metabolic pathway in plants?

In my proteomic results, there is change in glycolysis and TCA and i want to know that which pathway is involved in changing these major metabolic processes. For that i want to do some more...

18 September 2016 396 3 View

How to get started building and analyzing a UTAUT model?

Hello all, I need help getting started building and analyzing a UTATU model. I have a survey ready with some questions but I had some confusions on UATAUT and on how to analyze the data. I will...

02 March 2021 9,271 1 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

Which type of loading is to be used in dynamic analysis of Machien foundation?

Hello Everyone, Hope you all are doing well. I'm trying to simulate a machine foundation in ABAQUS with a geo-cell reinforced soil bed. Im totally confused to which type of loading would be...

21 February 2021 4,388 3 View

When to use different framework?

Hi, can I use both conceptual framework and theoretical framework in my systematic review? My future study plan is to use a theory. In this situation for my systematic review should I use both or...

18 February 2021 9,581 2 View

Which test should I use in SPSS if I have two independent variables and one dependent variable that is measured using two tests?

I am researching the effect of gender and video game use on reaction times. To do this, I will have participants complete two reaction time tests (a simple test and a four choice test). I am a...

17 February 2021 9,343 4 View

How to judge and prove the uniqueness of the solutions to the following two matrix equations?

It is about the matrix equation AX=B, where a, b c in A and B are independent elements, X should be made of constants. Case 1 A=[a b 0;0 0 c]; B=[a b 0 0;0 0 c c]; The solution is X=[1 0 0 0;0 1...

16 February 2021 5,698 3 View

Choice of Ab for Co-IP: Does an anti-(tag) antibody (such as anti-flag/ anti-myc) a must if I am using co-transfected cells for Co-IP?

Hi guys, I am trying to detect the binding of 2 proteins by Co-immunoprecipitation. However, the choice of antibody is confusing me. I tried probing the endogenous protein right away but the...

16 February 2021 9,050 5 View