Need suggestions regarding effect size estimation and interpretation ?

My research paper takes into account the antecedents and consequences of Employee Wellbeing. It has 3 independent variables and 2 dependent variables. It also has 2 non-categorical moderator...

06 December 2020 1,196 3 View

Relation betwen tool and plate in frw process

09 February 2017 1,841 2 View

Has anyone immunized mice with Influenza HA1 antigen?

I would appreciate if anyone can share information about HA1 immunizations in mice. Particularly Cal09 HA1 in C57BL/6 mice or BALB/c mice. Any information about the murine MHC class2 restriction...

07 June 2016 3,665 3 View

What is the best way to identify protein in FPLC fractions?

I subject NiNTA purified proteins to size exclusion chromatography to get rid of any impurities and identify monomers. I'm not entirely sure what the best approach is for further downstream...

01 March 2016 8,761 5 View

What are the advantages of detecting memory B cells using ELISPOT instead of flow cytometry?

I plan to do an immunization and look at memory B cells among other B cell subsets. I will be doing a flow cytometric analysis (I will use cell surface markers and also look at antigen binding...

26 September 2015 7,402 3 View

Does anyone have experience looking at t follicular helper cells and b cells using flow cytometry?

I stained mouse lymph nodes with pd-1,cxcr5,cd4 and cd44 for t follicular helper cells and cd19,cd38,cd73 and af488 conjugated to an antigen that I originally immunized the mice with. I collected...

22 March 2015 6,336 1 View

Why is capture ELISA assay not detecting not working or capture HIS-tagged GP120?

21 March 2015 7,090 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Antibody anti-IgM Bovin conjugated Biotin ?

Hi everybody, Can anyone please suggest me an antibody anti-IgM Bovin conjugated Biotin ? I would like to use it in LUMINEX with a streptavidin conjugated phycoerythrin reporter. Thanks in...

25 February 2021 9,659 2 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

What is the best method to evaluate and measure non-circulating estrogen levels in mice ?

Could anyone suggest me the best procedure to measure the circulating estrogen levels in mice ? I came across the general methods where it can be measured in serum or urine but a detail suggestion...

22 February 2021 4,352 2 View

Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View

Hi..how to isolate crude HDL (all subfractions) from human serum by size exclusion chromatography?

I would like to know about isolation of crude HDL from serum by SEC. Is it possible to perform with a serum sample volume of

19 February 2021 5,576 2 View