Let's say you have designed new drugs (synthetic), and you tested them by invitro enzymatic assay or by MTT assay on normal cells such as fibroblasts of MCF10 or Hela cells... and you also tested them on cancerous cells, and regardless by which mean you tested their activity and toxicity; you got an IC50 for them,.... how do we interpret or analyze our result, i mean; what does the ic50 reflects either on a normal or cancerous tissue if it was 5microMolar, 10microMolar or 20 or 50 or less than 10, and how does it affect or judgment that it is potent or efficient, or active but toxic or selective? Could you please support you kind reply with reference or arrticles , please,
Thanks a lot
Dear Sir , see this link
https://www.quora.com/How-can-you-use-an-IC50-value-to-determine-drug-efficacy
https://www.researchgate.net/post/How_can_I_calculate_IC50_values_to_drugs_that_does_not_reach_a_100_of_inhibitory_activity_of_viability_of_tumoral_cells_for_example
There is no specific IC50 value that can be used to judge whether a compound is active, toxic, etc. You have to take many factors into consideration.Here are some. (1) Is the compound soluble in the growth medium at the IC50 concentration? If not, the IC50 could be an artifact of compound insolubility. (2) How much growth inhibition is needed to get a useful effect in vivo? If essentially 100% growth inhibition is needed, then the concentration required is higher than the IC50. (3) Is it possible to achieve a sufficient concentration of the compound in vivo to achieve the necessary concentration to inhibit cell growth? (4) Is there a reasonably large margin between the therapeutic and toxic concentratoion? In other words, are the IC50s with cancer cells and normal cells sufficiently different? You may need 100% growth inhibition of cancer cells but you may not be able to tolerate even 1% growth inhibition of normal cells, so the separation of the IC50s would have to be very large. In the end, you are probably going to need nanomolar growth inhibition IC50 with cancer cells and unmeasurably high growth inhibition IC50 for normal cells. However, the starting lead compound for a medicinal chemistry program is usually not ideal, and much effort goes into improving its properties (solubility, toxicity, pharmacokinetics).
First of all you have to have an end product in order to calculate the IC50. After that you can titrate your reaction by using different concentration of your compound.vs the percentage of the product then draw and calculate the mid point of the our curve.
Dear MS
Drug molecules are classified as low active or high active regarding their binding activity (IC50 values) on target proteins. The half maximal inhibitory concentration (IC50) is a measure of the effectiveness of a substance in inhibiting a specific biological or biochemical function. You may try plotting the logOD versus log drug concentration. Find 50% of control cells OD value and that will be IC50%.
Please check also more informations in the papers:
-Use of Different Parameters and Equations for Calculation of IC50 Values in Efflux Assays: Potential Sources of Variability in IC50 Determination. AAPS J. 2014 Jan; 16(1): 172–180.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3889528/
-Classification of drug molecules considering their IC50 values using mixed-integer linear programming based hyper-boxes method. BMC Bioinformatics. 2008; 9: 411. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2572625/
Kind regards
Marius
Regards
Marius
the free radical scavenging properties of a substance will be assessed at different concentration and graph will be prepared from this graph we should calculate the IC50 oof substance
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