What is the best way of processing very large files in python?

I want to process a very large file, let's say 300 GB, with Python and I'm wondering what is the best way to do it. One limitation is that my algorithm needs the whole file in memory, so a...

15 January 2021 6,691 4 View

Has anyone compared supervised classification methods using google earth engine?

Good Morning! Has anyone compared supervised classification methods using Google Earth Engine? Could you comment on what are the criteria you used to decide on any method?

29 November 2020 544 4 View

How can I find the correlation coefficient between 1 categorical variable compared to several other variables?

Let's say that I have 8 proteins: A-H and I have protein expression data for each gene. For each gene, the expression number is based on the average of 20 cell lines expressing that gene. I also...

10 May 2020 3,261 1 View

Can anyone clarify the difference between IC50 and dose to me?

I guess I'm not understanding the concept well. If I treated cells (5000/well) with a range of doses from 6nM to 92nM (and this is an MTT assay and I've used Prism8), why is the IC50 only .9nM? I...

24 February 2020 7,044 2 View

Is it really necessary the clean-up step after PCR for digesting with DpnI?

I've read different protocols, starting for the QuikChange one, and there's no call for cleaning up after PCR. However, I did clean up as suggested by other researchers, so the polymerase doesn't...

12 November 2019 9,677 5 View

Is it really necessary the clean-up step after PCR for digesting with DpnI?

I've read different protocols, starting for the QuikChange one, and there's no call for cleaning up after PCR. However, I did clean up as suggested by other researchers, so the polymerase doesn't...

10 November 2019 5,183 5 View

Protocol for polymer stainning with Ruthenium tetroxiode?

I cannot find a comprehensive protocol for stainning polymers with Ruthenium tetroxide solution. I would like to know the instructions and safety precautions for stainning and handling of...

04 November 2018 7,875 2 View

Are you interested in publishing in the Energies Special Issue "Optimization of Biodiesel and Biofuel Process"?

You can find proper information of this Special Issue in the following link: https://www.mdpi.com/journal/energies/special_issues/Biodiesel_and_Biofuel_Process

29 October 2018 1,429 21 View

APC channel positive with unstained cells?

One of my cell lines shows up as positive in APC channel without any APC conjugated antibody by flow. I only have two colors which was stained separately so none of the samples have both APC and...

19 November 2017 5,171 3 View

How to determine the therapeutic indices for antimicrobial peptides?

I'm working with AMP's from scorpion venom and after determining the hemolytic activity, I woud like to be sure to determine properly the therapeutic index. Thanks

10 November 2017 740 0 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

No title

01 March 2021 3,905 5 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...

24 February 2021 3,823 2 View

How to handle studies which report perfect effect sizes in a meta-analysis using Pearson's r?

I'm in the process of updating a meta-analysis and in one of the new studies, effect sizes are reported as Tau-U. Two of the effect sizes are reported as Tau-U = 1.00. I am using Andy Field's...

24 February 2021 2,265 3 View

Sample size determination for non-parametric tests?

How do I run a a priori sample size determination for non-parametric tests, like the Mann-Whitney U-test and the Kruskall-Wallis or other non-parametric tests that accord with the unpaired t-test...

24 February 2021 166 6 View

How to determine a priori sample size for t-test with unequal means?

I need to conduct a a priori sample size determinations for t-test with unequal means, but I don't know the sizes of the groups nor the ratio of both groups... Can I do a sample size determination...

24 February 2021 8,289 3 View

What are alternatives to factor analysis for a small sample?

My sample size is too small to conduct a factor analysis. I created my own psychometric scale for measuring Effectiveness of Support, which contains 38 items (4 sub-scales) Im really struggling...

23 February 2021 9,287 12 View