I have a question about negative control of a transfection experiment using siRNA in cells. the expression of the target gene for knockdown is decreased in the negative control (Thermo). What is the cause? The positive control is knocking down without any problem.
Hi Yumi, have you checked what it looks like when you compare untransfected native cells with cells transfected with negative control siRNA?
Hi Simon. Thank you for your answer.
Confirmed. Target gene expression is down regulated in cells transfected with negative control compared to non-transfected native cells. GAPDH expression is down regulated in positive control, while no down-regulation of GAPDH is observed in native cells. Considering the possibility of off-targeting of negative control, I used two types of negatvie control, but both show decreased expression of target genes. What does this mean?
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