During the growth of iPS cells on feeder cells without adding bFGF, the iPS colonies loses its pluripotency and differentiate, my question is how we could identify the differentiated cell type?
Just like for ESC, you could use immunocytochemistry to detect specific markers for differentiated cells
graft your iPS into the kidney capsule or leg intramuscularally into nude mice to generate teratoma. Section the teratoma and stain the sections with H&E and you can identify the type of cells your"iPSC" can generate.
Hi Waleed,
I am assuming that you are worried about the differentiation of the iPSC to other unknown kind of cell phate by losing their pluripotency. In that case just by observing the iPSC or ES cell colonies under light microscope or phase contrast microscope you can see some colonies are having some yelowish or blackish things are comming on as like piles. That can be the possibly differentiated iPSC. Or in other case some cells in the iPSC colony dont look verry compact and shows somewhat like flatterened structures or concave structure. That can be the possible signs of the differentiated iPSC.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line