Ravindra Kailasrao Zirmire

7 Questions 19 Answers 0 Followers

Questions related from Ravindra Kailasrao Zirmire

How can I do live cell imaging of actin dynamics with maximum resolution and maximum time by keeping the actin filaments in focus?

I am very new to the live cell imaging of actin. I am trying to visualise actin filament dynamics by transfecting Lifeact GFP by spinning microscope. I need to visualise the actin filament...

06 October 2017 7,692 4 View

Hi all, Can some one give some suggestion about doing live cell imaging of actin in human fibroblasts by lifeact GFP?

I am trying to visualise actin by lifeact GFP by perkin elmer spinning disc microscope,but for some reason my transfected cells are dying. I have added 100uM HEPES in the media and using...

01 October 2017 7,905 4 View

What concentration of agarose can be used for the mouse skin agarose embeding for vibrotome sectioning?

I am trying to do the vebrotome sectioning of the mouse skin.But at 4% agarose concentration sectioning is not working well.

13 May 2015 6,454 1 View

What stiffness of polyacrylamide based gel can one use to mimic the human neonatal heart matrix stiffness when culturing hESc derived cardiomyocytes?

We are culturing hESc derived cardiomyocytes on different substrate of different stiffness to study the effect of matrix stiffness on cytoskeleton organization. I would like to know what stiffness...

04 April 2015 8,465 1 View

What can be the possible reasons for the increased thickness of the skin dermis after wound closure?

We are using some chemicals to accelerate wound healing response and we saw some of them are accelerating the wound healing response but the dermal thickness is double than the unwounded skin.

14 November 2014 6,357 12 View

How can we distinguish the normal fibroblast and senescent fibroblast in the culture?

Can we distinguish the normal and senescent fibroblast in culture from their morphology? If during the prcess of trypsinisation if they are not getting trypsinised then can these fibroblast be...

22 October 2014 7,507 15 View

Can anyone give me an idea about what is the tissue stiffness of the human heart at different developmental stages from foetal to adult stage is?

In a human heart according to the increase in the developmental stage, the heart tissue stiffness (Extra cellular matrix stiffness) goes on increasing. Has anyone checked the human heart tissue...

10 April 2014 196 4 View