Waleed Osman

11 Questions 12 Answers 0 Followers

Questions related from Waleed Osman

Seeking Software Recommendations for SELEX NGS Data Analysis?

I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed

31 July 2024 1,196 5 View

What is the optimum Aptamer copy Number in the synthetic DNA library (for SELEX)?

Hello, I would like to know the optimum number for Aptamer copy (each unique sequence) that we can design in a synthetic DNA library (for SELEX). I found some companies provide the library with...

22 November 2021 4,903 0 View

Extra protein bands after recombinant protein purification-any thoughts?

When I overexpressed my recombinant protein in E.coli, i found multiple proteins overexpressed, and became more clear after protein purification using talon metal affinity resin column (Purified-...

31 October 2014 6,023 14 View

What is the most convenient medium condition for culturing Mouse iPS Cells on Feeder Cell Free System?

To keep the Pluripotency of Mouse iPSCs under feeder cells free culture, please share your experience.

14 July 2014 6,067 3 View

How can I count iPS cells?

When I use the traditional method for cell counting using Trypan blue, if found that human iPS cells are forming clusters, what is the better method for iPS cell counting?

30 May 2014 3,672 4 View

Fibroblast aggregation - any thoughts?

I would like to ask about the ability of fibroblast to form cell clusters. Please check the attached picture and give me your comment.

30 April 2014 8,672 5 View

For pluripotent markers expression, how long does it take after cell differentiation to be reduced?

Pluripotent markers expression after differentiation

25 December 2013 6,980 2 View

iPS differentiation.

We are using small molecules to induce iPS differentiation. The first photo show alkaline phosphatase screening for control iPS and after treating iPS with small molecule. 2nd and 3rd photos...

02 December 2013 5,205 6 View

Any advice on waking up iPSC to feeder free culture?

Is it possible to wake up iPSC stock in liquid nitrogen directly to feeder free culture or do I have to first wake it up on feeder dependent culture, and then change to a feeder free system?

29 November 2013 8,157 18 View

Can anyone advise regarding iPS feeder free culture?

I'd like to shift from iPS feeder dependent culture to feeder free culture. Some companies recommend to use a special medium for feeder free system. However, I know some researchers are using same...

29 November 2013 5,896 1 View

How could I identify the differentiation fate of iPS Cells?

During the growth of iPS cells on feeder cells without adding bFGF, the iPS colonies loses its pluripotency and differentiate, my question is how we could identify the differentiated cell type?

05 November 2013 7,299 3 View