7 Questions 9 Answers 0 Followers
Questions related from Waleed Osman
I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed
31 July 2024 1,026 5 View
Hello, I would like to know the optimum number for Aptamer copy (each unique sequence) that we can design in a synthetic DNA library (for SELEX). I found some companies provide the library with...
22 November 2021 4,870 0 View
When I overexpressed my recombinant protein in E.coli, i found multiple proteins overexpressed, and became more clear after protein purification using talon metal affinity resin column (Purified-...
31 October 2014 5,989 14 View
To keep the Pluripotency of Mouse iPSCs under feeder cells free culture, please share your experience.
14 July 2014 6,035 3 View
When I use the traditional method for cell counting using Trypan blue, if found that human iPS cells are forming clusters, what is the better method for iPS cell counting?
30 May 2014 3,635 4 View
We are using small molecules to induce iPS differentiation. The first photo show alkaline phosphatase screening for control iPS and after treating iPS with small molecule. 2nd and 3rd photos...
02 December 2013 5,169 6 View
Is it possible to wake up iPSC stock in liquid nitrogen directly to feeder free culture or do I have to first wake it up on feeder dependent culture, and then change to a feeder free system?
29 November 2013 8,125 18 View
