Question:Did somebody used pSPYCE/pSPYNE vectors doing BiFC assays?

Somebody who knows why the two empty vectors I used as negative control, the fluorescence I can see under fluorescence microscope.

10 November 2017 4,029 7 View

How can I study the promoters evolution?

07 August 2014 6,015 3 View

Which methods is better for divergence time estimates?

Divergence time estimates are important to evolution research, but different estimated methods have various results. So I don't know which strategy of estimate is better and commonly used in...

06 July 2014 1,405 10 View

Can anybody help with a problem of molecular docking and autodock software?

When I used autodock to dock a protein and a molecular, a problem occured. The structure of small molecular is Fig A, but when I opened in autodock, it changed like Fig B. I don't know what...

04 May 2014 3,028 6 View

How can I calculate entropy of a protein-ligand?

I want to know the entropy of a protein-ligand, how can I calculate it? Is there a software or package?

04 May 2014 2,611 2 View

How can I calculate natural evolution strategies (NES)?

04 May 2014 4,637 1 View

Which is the better tool or web server for protein structure prediction or homology modeling?

When I used I-TASSER, SWISS-MODEL, modeller homology modeling for my protein sequence, the results are much different, so I don't know which is the better. Anyone can give me some advice?

04 May 2014 4,696 3 View

How to use LIGPLOT software build a diagram like this?

I have a pdb file of a protein and a small molecules, respectively and I want to get a diagram like this figure, but I don't know how can I do it. I ask for your help if you have used this software.

04 May 2014 8,094 8 View

How can I discover SNPs from the upstream and downstream of gene?

I want to count all SNPs from the upstream and downstream of a gene and I have a reference genome sequence and all upstream and downstream sequence of the genes. However, I don't know how to...

02 March 2014 7,416 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

How can I set up a good fit SEM model in AMOS?

I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...

26 February 2021 8,642 4 View

How to plot the probability paper of Gumbel distribution using R programing?

In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...

25 February 2021 5,229 3 View