Question:Did somebody used pSPYCE/pSPYNE vectors doing BiFC assays?

Somebody who knows why the two empty vectors I used as negative control, the fluorescence I can see under fluorescence microscope.

10 November 2017 4,029 7 View

How can you read .cel files using the R program?

10 November 2014 9,671 1 View

How can I study the promoters evolution?

07 August 2014 6,015 3 View

Which methods is better for divergence time estimates?

Divergence time estimates are important to evolution research, but different estimated methods have various results. So I don't know which strategy of estimate is better and commonly used in...

06 July 2014 1,405 10 View

Can anybody help with a problem of molecular docking and autodock software?

When I used autodock to dock a protein and a molecular, a problem occured. The structure of small molecular is Fig A, but when I opened in autodock, it changed like Fig B. I don't know what...

04 May 2014 3,028 6 View

How can I calculate natural evolution strategies (NES)?

04 May 2014 4,637 1 View

Which is the better tool or web server for protein structure prediction or homology modeling?

When I used I-TASSER, SWISS-MODEL, modeller homology modeling for my protein sequence, the results are much different, so I don't know which is the better. Anyone can give me some advice?

04 May 2014 4,696 3 View

How to use LIGPLOT software build a diagram like this?

I have a pdb file of a protein and a small molecules, respectively and I want to get a diagram like this figure, but I don't know how can I do it. I ask for your help if you have used this software.

04 May 2014 8,094 8 View

How can I discover SNPs from the upstream and downstream of gene?

I want to count all SNPs from the upstream and downstream of a gene and I have a reference genome sequence and all upstream and downstream sequence of the genes. However, I don't know how to...

02 March 2014 7,416 2 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View