Question:Did somebody used pSPYCE/pSPYNE vectors doing BiFC assays?

Somebody who knows why the two empty vectors I used as negative control, the fluorescence I can see under fluorescence microscope.

10 November 2017 4,029 7 View

How can you read .cel files using the R program?

10 November 2014 9,671 1 View

How can I study the promoters evolution?

07 August 2014 6,015 3 View

Which methods is better for divergence time estimates?

Divergence time estimates are important to evolution research, but different estimated methods have various results. So I don't know which strategy of estimate is better and commonly used in...

06 July 2014 1,405 10 View

Can anybody help with a problem of molecular docking and autodock software?

When I used autodock to dock a protein and a molecular, a problem occured. The structure of small molecular is Fig A, but when I opened in autodock, it changed like Fig B. I don't know what...

04 May 2014 3,028 6 View

How can I calculate entropy of a protein-ligand?

I want to know the entropy of a protein-ligand, how can I calculate it? Is there a software or package?

04 May 2014 2,611 2 View

How can I calculate natural evolution strategies (NES)?

04 May 2014 4,637 1 View

Which is the better tool or web server for protein structure prediction or homology modeling?

When I used I-TASSER, SWISS-MODEL, modeller homology modeling for my protein sequence, the results are much different, so I don't know which is the better. Anyone can give me some advice?

04 May 2014 4,696 3 View

How to use LIGPLOT software build a diagram like this?

I have a pdb file of a protein and a small molecules, respectively and I want to get a diagram like this figure, but I don't know how can I do it. I ask for your help if you have used this software.

04 May 2014 8,094 8 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View

Please suggest me the simplest way to understand the in-silico analysis of any gene?

I want to understand the basics of docking, and also know about all bioinformatics tools which are free of coast and helpfull in in-silico analysis?

22 February 2021 8,879 2 View

Is there a Constitutive model for concrete with Sea-Sand?

Hi I am running a simulation in Abaqus for a concrete beam. Its fine aggregate replaced with sea-sand. I was wondering if I could find studies that measured the ultimate strain of concrete made...

22 February 2021 6,409 2 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View