Divergence time estimates are important to evolution research, but different estimated methods have various results. So I don't know which strategy of estimate is better and commonly used in research or which tools are best used?
I agree with Ortaç Çetintaş that BEAST is a good place to start.
In any estimation process I think it is a good idea to use several different independent methods - every tool will give a different answer, and all will be wrong, but you will then have a distribution of estimates given by the different tools, and that gives you a better answer I think.
Divergence time estimation can be done through BEA Uti and BEAST here is the link for tutorial, just follow the tutorial and then go with your samples.
(Download BEAST, http://beast.bio.ed.ac.uk/)
(For tutorial and help: http://ib.berkeley.edu/courses/ib200/labs/ib200_lab09_BEAST.pdf)
Also you will need Tracer to analyze traces and Fig tree to view phylo. tree (find these softwares here, in latest softwares section: http://beast.bio.ed.ac.uk/).
Hi, guys. I am working with RelTime these days. But the result report negtive value about CI_Lower divtime. What's wrong with my settings? Is my fossils calibrations inaccurate?
I'm following the tutorial but missing something... I need to use volcanic island calibration of 0.5Ma but what do I do in Tips? All other tutorial already have dates assigned to the end of the sequences so that's fine but what about what I need to do? Confused.