We generated clonal cell lines from p48-Cre X mutKras/mutp53 mouse lines and use these lines as allografts. Due to the strain mix in the donor line, we expected and do see some rejection of the allografts in some recipients even when those are bred from the same ancestors.
Question: What would be the best test to assess the tolerance of potential hosts ahead of time ? If there are any published references or a service provider for the tests that would be great. Thank you.
Anton Wellstein, Georgetown University, Washington DC
H-2 haplotype/allotype matching will allow for prediction from the Major Histocompatibility Complex but will not prevent rejection due to minor histocompatibility mismatches outside of the MHC.
1. in vitro, The mixed leukocyte reaction assay can be used to evaluate the recipient response to donor cells from 48-Cre X mutKras/mutp53 mouse lines
2. in vivo, skin graft assay also can be used to examine!
Shiu-Huey,
thank you for your response ! Do you have a protocol or publication with a protocol that could guide us when we set this up ?
Best wishes
Anton Wellstein e-mail: [email protected]
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