Technique to detect immune infiltrates in mouse tissue sections. Preferably formalin-fixed, paraffin-embedded (FFPE).
Does anybody have a protocol and successfully isolated, grown and expanded cancer cells from leukapheresis of patients with solid tumors such as breast, colon, pancreatic, lung cancer ? Lombardi...
31 December 2014 10,133 1 View
We generated clonal cell lines from p48-Cre X mutKras/mutp53 mouse lines and use these lines as allografts. Due to the strain mix in the donor line, we expected and do see some rejection of the...
08 September 2014 8,839 3 View
We generated PCR products of different lenghts ( 200 and 2,000 nts ) from different types of assays: RACE-PCR, cDNA library inserts, amplicons from mutated DNA sites in tumors with heterogeneous...
07 August 2014 6,748 9 View
We wish to recover cells from a brain tumor specimen that were frozen at the time of surgery and wonder if someone has a suggestion on a protocol.
03 April 2014 1,877 1 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 454 3 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...
04 August 2024 4,130 4 View